摘要目的：利用代谢组学的方法发现与人辐射损伤密切相关的特征血浆代谢物，初步探讨辐射损伤涉及的代谢通路。方法收集2012年1月至2014年5月期间40名患者全身照射（TBI）辐照前后的血浆样本，通过气相色谱-质谱（GC-MS）联用的代谢组学方法研究TBI对人血浆代谢物的影响，并筛选出与TBI损伤密切相关的特征血浆代谢物。结果 TBI后，血浆中葡萄糖、肉豆蔻酸、草酸、3-羟基丁酸、尿素、天冬氨酸、缬氨酸、亮氨酸、赖氨酸和苏氨酸含量显著增加（P＜0.05），胆固醇、丙酮酸、丙酸、乳酸、丙氨酸、甘氨酸、肌醇、山梨糖酐、乙二醇和次黄嘌呤含量显著降低（P＜0．05）。结论 TBI可引起人体血浆中代谢物水平显著变化，主要涉及氨基酸代谢、糖代谢、脂代谢等代谢通路。

abstractsObjective To investigate radiation-related human plasma metabolic features by using metabonomics method and to analyze relative metabolic pathway .Methods The plasma samples of 40 patients pre-and post-total body irradiation (TBI) from January 2012 to May 2014 were collected, and the effect of TBI on human plasma metabolites was studied by gas chromatography mass spectrometry ( GC-MS) , and the differential plasma metabolic features related to irradiation damage were screened . Results The levels of glucose, myristic acid, oxalic acid, 3-hydroxy butyric acid, urea, aspartic acid, valine, leucine, lysine and threonine in plasma were significantly (P<0.05) increased after TBI, while the levels of cholesterol, pyruvic acid, propionic acid, lactic acid, alanine, glycine, inositol, sorbitan, ethylene glycol and hypoxanthine were decreased drastically (P<0.05).Conclusions TBI could cause significant changes in the levels of human plasma metabolites including amino acid metabolism , glucose metabolism, lipid metabolism and so on.