摘要目的 探讨电磁脉冲(EMP)对雄性小鼠生殖的影响.方法 48只健康成年雄性BALB/c小鼠(8周龄),用随机数表法分为假暴露组和暴露组,每组24只.动物进行EMP全身暴露或假暴露,EMP场强720 kV/m,脉宽40 ns,重复频率1 Hz,脉冲次数100次.EMP暴露后不同时间(1、7、14和35 d)腹腔麻醉小鼠,剥离双侧附睾尾,游离精子,检测精子质量(包括精子数量、畸形率和存活率);剥离双侧睾丸,HE染色法观察其组织形态,测量生精小管直径;ELISA和Western blot检测睾丸组织内干细胞因子(SCF)和胶质源性神经营养因子(GDNF)的水平.结果 EMP暴露后1、7、14和35 d,与假暴露组相比,暴露组小鼠的精子质量和睾丸形态结构无明显改变(P＞0.05);小鼠生精小管直径,暴露组分别为(196.85±16.65)、(196.79±14.33)、(196.35±22.71)和(198.60±25.88) μm;假暴露组分别为(204.31±27.13)、(197.07±18.11)、(194.37±21.45)和(200.59±19.36) μm,两组比较差异无统计学意义(P＞0.05).SCF和GDNF水平在EMP暴露后不同时间差异无统计学意义(P＞0.05).结论 本实验条件下的EMP暴露对成年雄性小鼠的睾丸结构和生殖功能无明显影响.

abstractsObjective To investigate the effects of electromagnetic pulse (EMP) on reproductive function of male adult mice.Methods A total of 48 healthy adult male BALB/c mice (8 weeks old) were randomly divided into sham group and EMP group with 24 animals in each group.The mice were wholebody exposed or sham exposed to EMP at 720 kV/m for 100 pulses with 1 Hz repetition rate and 40 ns pulse width.At 1,7,14 and 35 d after EMP exposure,the mice were anesthetized and the sperms were collected from the bilateral epididymal tail.After that,the sperm quality including the number of sperms,the ratio of abnormalities and the survival rate was evaluated.In addition,the morphology of testis was observed by HE staining and the diameter of seminiferous tubules was measured by Image J 1.43 u software.The protein level of stem cell factor (SCF) and glial-derived neurotrophic factor (GDNF) in testis tissue were detected by ELISA and Western blot.Results The sperm quality and the morphology of testis did not change obviously at different times after exposing mice to EMP at 720 kV/m for 100 pulses,compared with sham group (P＞0.05).The diameters of seminiferous tubules at 1,7,14 and 35 d after exposure were (196.85+ 16.65),(196.79+ 14.33),(196.35±22.71) and (198.60±25.88) μm in exposed mice,respectively,while (204.31±27.13),(197.07± 18.11),(194.37±21.45) and (200.59± 19.36) Iμm in sham exposed mice,respectively.There was no significant difference between two groups (P＞0.05).Additionally,the levels of SCF and GDNF in testis tissue between EMP group and sham group had no statistically significant difference (P＞0.05).Conclusion Under this exposure condition,EMP couldn't affect the reproductive function of male adult mice.