摘要目的 检测环氧合酶-2、低氧诱导因子-1α和血管内皮生长因子在胰腺癌组织中的表达情况.并进一步研究选择性环氧合酶-2抑制剂尼美舒利对胰腺癌BxPC-3细胞株中这3种蛋白表达的影响.探讨环氧合酶-2在胰腺恶性肿瘤血管生成过程中的作用及意义.方法 应用Western blot-ting检测12例胰腺癌及相应癌旁组织中环氧合酶-2、低氧诱导因子-1α和血管内皮生长因子蛋白的表达情况,应用不同浓度(分别为DMSO组.50 μmol/L,100 μmol/L组和200 μmol/L组)尼美舒利作用于BxPC-3细胞24 h后.提取细胞蛋白行Western blotting.观察3种蛋白表达水平的变化.结果 12例胰腺癌组织标本中均检测到有环氧合酶-2蛋白的高表达.相比之下,癌旁组织中则相对低表达甚至未表达.低氧诱导因子-1α和血管内皮生长因子蛋白也存在相同情况,即在癌组织中的表达要明显高于癌旁组织.而在尼美舒利不同浓度作用于胰腺癌BxPC-3细胞后,可明显抑制3种蛋白的表达水平(P<0.01),并呈剂量依赖性.结论 胰腺癌组织中环氧合酶-2、低氧诱导因子-1α和血管内皮生长因子呈过量表达.提示环氧合酶-2可能与胰腺肿瘤血管生成有关,而选择性环氧合酶-2抑制剂尼美舒利可抑制这3种蛋白的表达水平,这可能为其抗肿瘤新生血管形成的机制之一.

abstractsObjective To detect the expression of cyclooxygenase-2(COX-2),hypoxia induc-ible factor-1 alpha(HIF-1α)and vascular endothelial growth factor(VEGF)in pancreatic carcinoma,investigate effect of nimesulide,a COX-2-selected inhibitor,of various concentrations on the expres-sion of the 3 proteins and explore the relation of COX-2 expression to angiogenesis.Methods The ex-pression of COX-2,HIF-1α and VEGF in 12 cases of pancreatic carcinoma and corresponding paracar-cinoma tissues were detected by western blotting.The effect of nimesulide of different concentrations on expression of the 3 proteins was also determined by Western blotting.Results The expression lev-el of the 3 proteins was significantly higher in the pancreatic carcinoma than in paracarcinoma tissues.Meanwhile,nimesulide of various concentrations significantly reduced the expression level of the 3 proteins.Conclusion Over-expression of COX-2 might be responsible for increase of H IF-1α and VEGF production in pancreatic carcinoma,which suggests that COX-2 may be related to tumor angio-genesis.Furthermore,COX-2-selected inhibitor nimesulide can inhibit expression of HIF-1α and VEGF in pancreatic carcinoma.