摘要目的 研究胆囊胆固醇结石病人肝脏的肝脏受体类似物1(Liver receptor homolog 1,LRH-1)及其调控的三磷酸腺苷结合盒(ABC)转运体家族成员abcg5/8的表达,探讨胆固醇结石病发病的机制.方法 27例胆囊胆固醇结石病人,男6例,女21例;年龄平均52岁.10例无胆石症的胆囊息肉病人为对照,男6例,女4例;平均年龄48岁.测定胆汁脂类成分和计算胆汁胆固醇饱和指数.实时定量PCR法测定肝脏LRH-1及abcg5/8 mRNA的表达量.结果 胆石组LRH-1表达高于对照组(14.18±1.80vs7.22±2.22,P<0.05),胆石组肝脏abcg基因mRNA表达量均较对照组增高(abcg5:49.34±3.68 vs 33.48±2.77,P<0.05)abcg8:38.93±5.70 vs 18.70±3.42,P<0.05).胆石组胆汁呈胆固醇过饱和(1.17±0.02).结论 该研究结果提示人类肝脏LRH-1及其调控的abcg5/8的表达增高与胆囊胆固醇结石形成有关.

abstractsObjective To investigate the mRNA expression of liver receptor homolog 1(LRH-1) gene and abcg5/8 genes regulated by LRH-1 in patients with cholesterol gallstone disease to eluci-date the biomolecular pathogenesis of gallstone formation. Methods Twenty-seven patients with cho-lesterol gallstone(CGS) and 10 controls without gallstones were enrolled in this study. The biliary composition was assayed, mRNA expression of LRH-1 and abcg 5/8 genes was determined by RT-PCR. Results The expression levels of LRH-1 and abcgS/8 genes were significantly higher in CGS patients than in controls(LRH-1:14.18±1.80 vs 7. 86±2.22; abcg5:49.34±3.68; abcg8:38. 93±5.70 vs 18. 70±3.42; P<0. 05). Conclusion CGS disease may be related to increased expression of LRH-1 and abcg 5/8 genes.