摘要目的 研究氯磷酸二钠脂质体对急性坏死性胰腺炎肺泡巨噬细胞凋亡影响.方法 将8只急性坏死性胰腺炎SD大鼠利用肺泡灌洗液.经差时贴壁法,分离、纯化肺泡巨噬细胞,并进行原代培养,分别取5孔分为ANP对照组、ANP+空白脂质体组(50 μ1,100 μ1)、ANP+氯磷酸二钠脂质体组(50 μ1,100 μ1),用MTT比色法检测大鼠肺泡巨噬细胞光密度值、吖啶橙荧光染色、苏木精染色对所分离的细胞进行凋亡鉴定.结果 加入空白脂质体为50,μ1、100μ1时对肺巨噬细胞无显著差异(P＞0.05).当加入氯磷酸二钠脂质体为50 μ1/ml时有显著性差异(P＜0.05)、100μ1时亦有显著性差异(P＜0.01).细胞荧光染色和苏木精染色可以明确细胞凋亡情况.结论 脂质体包裹氯磷酸二钠可以诱导大鼠肺泡臣噬细胞凋亡.

abstractsObjective To study the effects of elodronate-liposome on inducing apoptosis of alve-olar macrophages from rats with acute neetotizing pancreatitis (ANP).Methods The AMs of eight rats with ANP were isolated, purified then incubated from broehoalveolar lavage by the differing rates of attachment of the various cell types in a forty-well cell culture plate.Then they were randomized in-to five groups including control group,blank liposome group( 50 μ1, 100 μ1),clodronate-liposome group (50μ1,100μ1).Values of OD were determined by MTT.AO fluorescence and haematoxylin dye were employed to determine the apoptosis of the AMs.Results There were no significant differences be-tween control group and blank liposome group(50 μ1, 100 μ1).Significant differences were found be-tween control group and clodronate-liposome group(50 μ1, 100 μ1).There were no marked differences between blank liposome group(50μ1, 100 μ1)and clodronate-liposome group(50 μ1,100μ1).AO fluo-rescence and haematoxylin dye were available to define the apoptosis of the AMs.Conclusion Clodr-onate-liposome can effectively induce the apoptosis of the AMs.