摘要目的 我国是乙型肝炎病毒感染大国.乙型肝炎病毒(hepatitis B virus,HBV)的持续感染及复制与肝癌关系密切,是原发性肝癌的主要病因之一.病毒特异的小干扰RNA(siRNA)是抑制病毒复制和蛋白表达的强大武器.本研究旨在了解siRNA抑制HBV表达及复制对感染HBV的肝癌细胞增殖的影响,找到对抗肝癌的有效方法.方法 将siRNA转入含HBV病毒基因的肝癌细胞系HepG2.2.15中,测定上清中HBsAg表达及HBV DNA拷贝数;用MTT法评估其增殖能力.结果 笔者所选的特定的siRNA都能有效减少HBsAg分泌和HBV DNA拷贝数,其中HBsAg抑制率最高达83.9%,HBV DNA拷贝数抑制率最高达73.4%;MTT法发现抑制率最高的siRNA转染细胞后,第五天其增殖能力较空白组明显减弱,而其他组与空白组无明显差别.结论 特定的siRNA能有效减少HBV复制及蛋白表达;抑制HBV表达能抑制HepG2.2.15细胞系细胞的增殖能力.

abstractsObjective Virus-specific RNA interference (RNAi) is a powerful inhibitor of gene expression and replication of HBV. It is known to have high efficiency, specificity, and few side effects. We wanted to evaluate the effects of siRNA silencing HBV replication on the growth of hepatocellular carcinomatic(HCC) cells to find out an ideal method for treatment of HCC. Methods We transfected siRNA into HepG2.2. 15 cells (HCC cell inserting HBV gene) and detected the HBsAg and HBV DNA copies for evaluating the inhibitory effects of siRNA. Then we evaluated cell growth and self-renewal ability after transfection of siRNA by MTT. Results The HBsAg level and HBV DNA copies were reduced after the transfection of siRNA, the highest inhibition rate was 83.9%,while the inhibition rate of HBV DNA copies reached 73. 4%. The siRNA group's growth ability and self-renewal rate were lower than the control group in 5 days. Conclusion siRNA can effectively inhibit HBV replication and expression in HepG2.2.15 cells and silencing HBV replication can inhibit HepG2.2.15 cell's growth and self-renewal.