摘要目的 研究不同强度高压电场作用对人胰腺癌细胞株PANC-1细胞生长、凋亡以及细胞微结构的影响.方法 选择处于对数生长期的PANC-1细胞,将实验分为5个组进行研究:对照组细胞未施加电场处理,高压电处理组细胞分别施加250、500、750、1 000 V/cm场强的高压电场.通过细胞活率测定、细胞染色、Annexin V-PI凋亡检测、透射电镜以及扫描电镜等技术来研究不同强度高压电场对PANC-1细胞生长以及细胞微结构的影响.结果 在场强≥500 V/cm时,高压电场显著抑制PANC-1细胞生长呈场强依赖性,与对照组比较差异有统计学意义(P＜0.05).高压电场能诱导肿瘤细胞凋亡,在场强≥750 V/cm时,细胞坏死严重.与对照组相比,场强为1 000 V/cm实验组的细胞膜完整性以及细胞器结构损伤严重.结论 高压电脉冲能显著抑制PANC-1细胞生长,是一种极具应用前景的肿瘤治疗方法.

abstractsObjective To investigate the effects of high intensity electric field on cell growth,apoptosis and microstructure of human pancreatic cancer cell line PANC-1.Methods The PANC-1 cells in the logarithmic growth period were selected,and cells in the high voltage electrical treatment group were treated with high voltage electric field 250,500,750,1000 V/cm,respectively.The effects of different high voltage electric fields on cell growth and microstructure of PANC-1 cells were determined by cell viability,cell death staining,apoptosis detection,transmission electron microscopy and scanning electron microscopy.Results Compared with control group,the high voltage electric pulse significantly inhibited the growth of PANC-1 cells in the field dependent manner.Moreover,when the field was more than 500 V/cm,the cell viability was significantly decreased (P＜0.05).High voltage electric pulse could induce cell apoptosis.When the field was higher than 750 V/cm,serious necrosis was noticed.In the 1000 V/cm group,the integrity of cell membrane and the structure of organelles was seriously damaged.Conclusion High voltage electric pulse could significantly inhibit the growth of PANC-1 cells and would be a promising method in cancer treatment.