摘要目的 检测微RNA-1290在胰腺癌中的表达,探讨其在胰腺癌侵袭转移中的作用.方法 免疫组织化学技术检测胰腺癌组织芯片中微RNA-1290的表达,研究其在胰腺癌侵袭转移中的临床意义;实时定量-PCR技术检测5种胰腺癌细胞系AsPC-1、BxPC-3、Capan-2、Panc-1、MIA PaCa-2中微RNA-1290的表达;用微RNA-1290抑制剂处理对数生长期的胰腺癌细胞系Panc-1和MIA PaCa-2,并用Transwell和细胞划痕实验技术检测胰腺癌细胞的侵袭转移能力;蛋白印记(westem blot)检测胰腺癌细胞系中侵袭转移相关蛋白COX-2、MMP-2的表达.结果 (1)胰腺癌组织中微RNA-1290的表达显著高于正常胰腺组织以及癌旁组织(均P＜ 0.05).(2)与胰腺正常上皮细胞HPDE相比,不同胰腺癌细胞系中微RNA-1290的表达量均显著升高(均P＜0.05),且微RNA-1290在Panc-1和MIAPaCa-2胰腺癌细胞中的表达量显著高于其他胰腺癌细胞系(均P＜0.05).(3)微RNA-1290抑制剂处理胰腺癌细胞Panc-1和MIA PaCa-2后,其胰腺癌细胞的侵袭和转移能力均显著降低(均P＜0.05).(4)微RNA-1290抑制剂能抑制胰腺癌Panc-1和MIA PaCa-2细胞的侵袭相关蛋白基质金属蛋白酶2(MMP-2)、环氧化酶2(COX-2)的表达.结论 胰腺癌细胞系和胰腺癌组织中微RNA-1290可能通过调节MMP-2、COX-2等基因的表达参与胰腺癌细胞的侵袭转移.

abstractsObjective To investigate the expression of microRNA-1290 in pancreatic cancer and its role in invasion and metastasis of pancreatic cancer.Methods The expression of microRNA-1290 in pancreatic cancer tissue microarray and pancreatic cancer cell lines (AsPC-1,BxPC-3,Capan-2,Panc-1,and MIA PaCa-2) were detected by immunohistochemistry and QT-PCR.The pancreatic cancer cell lines Panc-1 and MIA PaCa-2 in logarithmic growth phase were treated with microRNA-1290 inhibitor,and the invasion and metastasis ability of pancreatic cancer cells were detected by Transwell and wound healing asssay.Western Blot was used to detect the expression of invasion and metastasis-associated proteins cyclooxygenase 2 (COX-2) and matrix metalloproteinase 2(MMP-2) in pancreatic cancer cell lines.Results (1) The expression of microRNA-1290 in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues and adjacent tissues (P ＜ 0.05).(2) Compared with pancreatic normal epithelial cells (HPDE),the expression of microRNA-1290 was significantly higher in different pancreatic cancer cell lines (P ＜ 0.05).The expression level of MicroRNA-1290 in Panc-1 and MIAPaCa-2 pancreatic cancer cells was significantly higher than that in other pancreatic cancer cell lines (P ＜ 0.05).(3) The number of invasive and metastatic cells was significantly decreased after treatment with microRNA-1290 inhibitor (P ＜0.05).(4) The expression of MMP-2 and COX-2 were decreased in Panc-1 and MIAPaCa-2 pancreatic cancer cells treated with MicroRNA-1290 inhibitor.Conclusion The expression of MMP-2 and COX-2 may be involved in the invasion and metastasis of pancreatic cancer cell by regulating the expression of microRNA-1290 in pancreatic cancer.