摘要目的探讨癌基因rasp21、C-myc、C-erbB-2和AFP在2-乙酰氨基芴诱发的实验性大鼠肝癌变过程中的分布情况及关系。方法 rasp21、C-erbB-2、C-myc和AFP，采用免疫组织化学SP方法检测分析。结果诱癌早期小叶周边增生及变异的肝细胞灶中即显示rasp21、AFP和C-myc的表达，并随增生肝细胞结节的形成和演进，所表达的细胞也增多并且相伴而存。诱癌中期出现C-erbB-2的表达。结论癌基因蛋白rasp21、C-myc、AFP是大鼠实验性肝癌变中较早出现的分子改变，可能与肝癌的启动和演进有关，也是肝癌形态发生的分子基础之一。三者具有协同作用。而C-erbB-2的异常表达则可能对癌前期病变的发展具有促进作用。同时也表明肝细胞的恶性转化需要多个癌基因的协同作用。

abstractsObjective To investigate the distribution of oncogenes and their relationships in 2-FAA induced experimental hepatocarcinogenesis. Methods Rasp21, C-myc, C-erbB-2, and AFP were detected and analyzed by the SP method of immunohistochemistry. Results Some cells expressed rasp21, C-myc, and AFP in peripheral liver lobules and alternated hepatocyte foci in the early stage of the experiment. And with the formation and progression of hyperplastic hepatocytic nodules, expressed cells increased and often accompanied each other. Expression of C-erbB-2 appeared in the middle stage of the hepatocarcinogenesis. Conclusions Expression of rasp21, C-myc, and AFP in the experimental hepatocarcinogenesis is an early molecule change, which may relate to the initiation of hepatocarcinogenesis and be considered to be one of the molecular bases for the morphogenesis of hepatocarcinogenesis. During the development of lesion, C-erbB-2 may have promoting effects. It is also clear that malignant transformation of hepatocyte needs the cooperation of multiple oncogenes.