摘要目的 建立中国HBV B、C基因型参照序列.方法 从GenBank中获得来源于中国不同地区的HBV全基因序列.通过基因分型和序列比对,以相同位置频率最高碱基作为参照碱基建立序列;将这些参照序列与国内外的参照序列进行比较,了解其中的不同点,对不同编码区的氨基酸序列进行比较.对B,C基因型HBV的碱基替代情况进行统计学分析,其中1762、1764、1896位点采用χ~2检验,1858位点采用Fisher's确切概率法检验. 结果获得了中国HBV B,C基因型参照序列.Bc与Ba、Bj基因型HBV的全基因序列同源性分别为99.32%,95.52%,S基因序列同源性分别为99.71%、98.68%; Cc与C、Caus基因型的全基因序列同源性分别为98.44%、93.97%,S基因序列同源性分别为99.27%、95.01%.与Bj型相比.Bc和Ba型同源性更高;与Caus型相比,Cc和C型同源性更高.不同编码区的氨基酸序列也有差异;两种基因型在1762、1764、1858位点的碱基分布差异均有统计学意义(P值均<0.05).结论 建立的HBV B、C基因型参照序列可以作为中国HBV参照序列,为设计引物和分析碱基或(和)氨基酸变异提供参考.

abstractsObjective Toestablishthe reference sequences of genotype B and C of hepatitis B virus in China.Methods Genome sequences of Hepatitis B virus isolated from different area in China were retrieaved from GenBank.These genome sequences were alignmented and the most common sequences were regarded as reference sequences.The amino acid sequences were also alignmented.Results The homology was 99.32% between Bc genome and Ba genome,and it was 95.52% between Be genome and Bj genome.The S gene sequence homology was 99.71% between Bc and Ba,and it was 98.68% between Bc and Bj.The homology was 98.44% between Cc genome and C genome,and it was 93.97% between Cc genome and Caus genome.The S gene sequence homology was 99.27% between Cc and C,and it was 95.01% between Cc and Cans.There was significant difference at the sites of 1762,1764,1858 between genotype B and genotype C (P<0.05).The amino acid sequences were also different between genotype B and genotype C.Conclusion Bc and Cc sequences of Hepatitis B virus call be regarded as the reference sequences of genotype B and C.