摘要目的 观察温水浴复温与被动复温对长时程海水浸泡体温过低症SD大鼠肺脏病理损伤及动脉血气的影响.方法 100只雄性成年SD大鼠随机均分为正常对照组(不做任何处理),低温实验组(20℃海水浸泡24h),被动复温组1、2、3、4(20℃海水浸泡24 h+被动复温,分别在复温后0、3、6、12h处死),温水浴复温组1、2、3、4(20℃海水浸泡24 h+温水浴复温,分别在复温后0、3、6、12 h处死),每组10只.主要检测肺脏病理学及动脉血气等指标改变.结果 温水浴复温与被动复温对长时程海水浸泡体温过低症大鼠肺损伤及动脉血气异常均有恢复作用.与被动复温组相比,温水浴复温组大鼠在复温后6h、12h,肺脏病理损伤恢复更好,差异有统计学意义[温水浴复温组Smith病理评分:6h时为(15.8±1.2)分,12 h为(3.8±1.4)分;被动复温组6h为(7.6±2.2)分,12 h时为(5.3±1.3)分](P＜0.05);在复温后6h实际碳酸氢盐恢复更好,差异有统计学意义(P＜0.05).结论 与被动复温相比,温水浴复温对长时程海水浸泡体温过低症大鼠肺损伤及动脉血气异常的改善作用更明显,对防止复温相关的急性呼吸窘迫综合症的发生可能更加有效.

abstractsObjective To observe the effects of warm water bath active rewarming and passive rewarming on lung pathological injury and arterial blood gas of SD rats with hypothermia induced by prolonged seawater immersion.Methods One hundred male Sprague-Dawley rats were randomly divided into the normal control group (without any treatment) and the hypothermia group (seawater immersion at 20 ℃ for 24 h),The animals in the passive rewarming groups 1,2,3 and 4,each consisting of 10,had seawater immersion at 20 ℃for 24 h and received passive rewarming,and then they were respectively executed at 0,3,6 and 12 hours after rewarming,The animals in the active rewarming groups 1,2,3 and 4,each consisting of 10,underwent seawater immersion at 20 ℃ for 24 h and received active rewarming,and then they were sacrificed at 0,3,6and 12 hours after rewarming,Changes in lung pathology,arterial blood gas and other indicators were detected in all the animal groups.Results Both warm water bath active rewarming and passive rewarming all could help to restore lung injury and blood gas abnormality in rats with hypothermia induced by prolonged seawater immersion.Compared with that of the passive rewarming group [6 h:(7.6 ± 2.2) scores,12 h:(5.3 _± 1.3)scores],the recovery of lung pathological injury in warm water bath rewarming group was obviously better at 6 h(5.8 ± 1.2) scores and 12 h(3.8 ± 1.4) scores after rewarming,with statistical significance (P ＜ 0.05),and actual bicarbonate recovery was even better at 6 h after rewarming,also with statistical significance (P ＜0.05).Conclusions Compared with passive rewarming,warm water bath rewarming could significantly alleviate lung injury and arterial blood gas abnormality in hypothermic rats induced by prolonged seawater immersion,and it might produce even better effect on the prevention of rewarming-related acute respiratory distress syndrome.