摘要目的:探究高压氧治疗对支气管哮喘患者血清趋化因子和免疫T细胞的影响，并分析其与细胞色素氧化酶(COX)活性的关系。方法:选取2017年1月至2018年12月吉林大学南岭校区医院和吉林大学中日联谊医院急诊科收治的96例支气管哮喘患者作为研究对象，按随机数字表法分为对照组和观察组，每组各48例。对照组给予常规治疗，观察组在对照组基础上联合高压氧辅助治疗。比较治疗前后患者血清趋化因子和CD 4+T细胞中Th1、Th2的变化，同时分析高压氧治疗与细胞色素氧化酶(COX)活性的关系。 结果:观察组患者显效38例，对照组仅27例，观察组治疗总有效率为95.83%，显著优于对照组的85.42%，差异有统计学意义( P<0.05)。治疗后，观察组患者动脉血氧分压(PaO 2)、第1秒呼气量(FEV1)及FEV1/用力肺活量(FVC)均有显著改善，较对照组明显上升，差异有统计学意义( P<0.05)。观察组和对照组患者血清嗜酸性粒细胞趋化因子(eotaxin)和单核细胞趋化因子(MCP-1)含量较治疗前显著下降，且观察组血清趋化因子降低明显优于对照组，差异有统计学意义( P<0.05)。2组患者治疗后外周血Th1及Th1/Th2比值明显升高，Th2显著下降，且观察组患者免疫T细胞亚群分布优于对照组。Person相关性分析发现，eotaxin、MCP-1水平及Th2含量与COX活性负相关( r＝-0.635， P＝0.004； r＝-0.673， P＝0.002； r＝-0.737， P<0.01)，而Th1含量与COX活性正相关( r＝0.684， P＝0.01)。 结论:高压氧治疗可通过提高COX活性、促进有氧代谢，减少支气管哮喘患者血清趋化因子的表达，增强免疫功能，有助于改善预后。

abstractsObjective:To explore the effects of hyperbaric oxygen treatment on the serum chemokines and immune T cells in the patients with bronchial asthma, and to analyze the correlation between the treatment and the activity of cytochrome oxidase (COX).Methods:A total of 96 patients with bronchial asthma treated from January 2017 to December 2018 were selected as the research subjects, and were randomly divided into control group and observation group ( n=48). The control group was given the conventional treatment, while the observation group was additionally treated with the hyperbaric oxygen therapy. The changes of serum chemokines and the changes of Th1 and Th2 (subsets of CD 4+ T cells) in the two groups were compared before and after the treatment. The correlation between the hyperbaric oxygen therapy and the COX activity was also analyzed. Results:In the observation group, 38 cases were markedly improved, while only 27 cases in the control group were markedly improved. And the total effective rate of the observation group (95.83%) was significantly higher than that of the control group (85.42%) ( P<0.05). After the treatment, the partial pressure of oxygen (PaO 2), FEV1, FEV1/FVC of the patients in the observation group were significantly increased as compared with those in the control group with statistically significant differences ( P<0.05). The serum eotaxin and monocyte chemoattractant protein-1 (MCP-1) in both two groups after treatment were significantly decreased as compared with those before the treatment, and the serum chemokines of the observation group were sharply reduced compared with that of the control group ( P<0.05). After the treatment, the Th1 in the peripheral blood and the ratio of Th1/Th2 of patients in the two groups increased significantly, while the Th2 decreased significantly. Moreover, the distribution of immune T cell subgroups in the observation group was better than that in the control group. According to person correlation analysis, the levels of eotaxin, MCP-1, and Th2 were negatively correlated with COX activity ( r=-0.635, P=0.004; r=-0.673, P=0.002; r=-0.737, P<0.001), while the Th1 was positively correlated with COX activity ( r=0.684, P=0.001). Conclusion:Hyperbaric oxygen therapy can strengthen the immune function of the patients with bronchial asthma and improve the prognosis by enhancing COX activity and active aerobic metabolism, thus reducing the expression of serum chemokines.