摘要目的 通过比较临床分离的不同fimA型牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)菌株黏附和侵入KB细胞的能力,探讨不同fimA基因型Pg菌株之间致病力差异的原因.方法 应用培养法和抗生素保护法测定不同fimA型Pg菌株黏附和侵入KB细胞的能力,扫描电镜观察不同fimA型Pg对KB细胞的黏附情况.结果 所有菌株均可黏附和侵入KB细胞,黏附率为0.523%～37.125%,侵入率为0.017%～3.750%;各fimA型Pg菌株之间黏附和侵入KB细胞的能力差异无统计学意义(P>0.05);各株Pg之间、4株Ⅱ型菌株之间、5株Ⅳ型菌株之间,黏附和侵入KB细胞的能力差异有统计学意义(P<0.01).结论 Pg的fimA型与其对KB细胞的黏附和侵入能力无相关性,提示可能存在其他影响不同fimA型Pg致病力差异的因子.

abstractsObjective To compare the ability of adhesion and invasion to epithelial cells by Porphyromonas gingivalis ( Pg) strains with different fimA separated from Chinese. Methods Cultured method and antibiotic protection method were used to determine the adhesive and invasive ability of Pg with different fimA genetypes. The adhesion was observed by scanning electron microscope. Results All the strains adhered and invaded to KB cells, and the adhesion rate ranged from 0.523% to 37. 125% and invasive rate from 0. 017% to 3. 750%. The adhesive and invasive ability among different fimA genotypes showed no significant difference (P >0. 05).Conclusions There is no significant correlation between fimA genotype and ability in adhesion and invasion to KB cells.