摘要目的研究转化生长因子β1( transforming growth factor,TGF-β1)及胰岛素样生长因子Ⅰ（insulin-like growth factor-Ⅰ，IGF-Ⅰ）对颞下颌关节(TMJ)盘细胞自组装基体细胞外基质合成的影响。方法采用10、100 μg/L的IGF-Ⅰ（Ⅰ10、Ⅰ100组）及5、50μg/L的TGF-β1（T5、T50组）分别作用于TMJ盘细胞自组装基体,3、6周时观察自组装基体的大小、形态并测重；冰冻切片、天狼猩红（picro-sirius red）及免疫组化染色观察Ⅰ型胶原的分布，番红-O-固绿染色观察Ⅰ型胶原及糖胺聚糖分布。定量检测Ⅰ型胶原、糖胺聚糖含量、测定细胞数量。结果3周时T5、Ⅰ10组上调糖胺聚糖的分泌、细胞的增殖以及Ⅰ型胶原的合成，3周时Ⅰ10组Ⅰ型胶原（109.16±5.12 μg）的表达约是空白对照组（69.13±5.94 μg）的近2倍；空白对照组和Ⅰ10、Ⅰ100、T5、T5o组于第6周时分泌的基质含量及细胞的增殖数量均比第3周增多。结论IGF-Ⅰ、TGF-β1作用时间的延长对基体的生化合成具有上调作用，10μg/L的IGF-Ⅰ更适用于组织工程化TMJ盘细胞自组装基体的应用。

abstractsObjective To examine the effects of high and low concentrations of transforming growth factor( TGF ) β1 and insulin-like growth factor-Ⅰ (IGF-Ⅰ) on the extracelluar matrix synthesis of the self-assembled constructs of temporomandibular joint (TMJ) disc. Methods The experimental groups of self-assembled constructs were exposed to IGF-Ⅰ( 10, 100 μg/L ) and TGF-β1 (5,50 μg/L ), the control groups were not added with any growth factors. All groups were examined at 3 and 6 weeks for gross morphological, histological, and biochemical changes. Safranin-O/fast green staining was used to examine glycosaminoglyean(GAG )distribution, picrosirius red and immunohistochemical staining to observe type Ⅰ collagen distribution. Type Ⅰ collagen contents were tested by ELISA assay kit, GAG contents were measured by Blyscan GAG assay kit, and the cell numbers were quantified with a Picogreen reagent kit. Results The growth factor groups all upregulated the matrix synthesis of the self-assembled constructs compared with control groups. TGF-β1 (5 μ4g/L) and IGF-Ⅰ( 10 μg/L) were the two most potent concentration in increasing type Ⅰ collagen and GAG synthesis and cells proliferation. IGF-Ⅰ group( 10 μg/L) produced nearly 2 times ( 109. 16 ±5. 12 μg) as much type Ⅰ collagen as the control group(69. 13 ±5.94 μg) at 3 weeks. The matrix contents and the number ff the proliferated cells in control group and all GF groups at 6 weeks were more than those at 3 weeks. Conclusions IGF-Ⅰ( 10 μg/L) is the most beneficial growth factor and can be applied in tissueengineering stratigies of the temporomandibular joint dise. At the same time,the exposure time of growth factors is another key factor that affects matrix synthesis of TMJ disc constructs.