摘要目的 比较4种自酸蚀粘接剂牙本质粘接界面纳米渗漏及微观形态。方法 选取16颗无龋人离体第三磨牙，制备统一的牙本质玷污层，分别用4种自酸蚀粘接剂A( Adper Prompt)、B( iBond)、C(XenoⅢ)及D(SE Bond)按使用说明进行粘接处理，垂直粘接面切成0.9 mmx4.0 mm试件，避光贮存于氨化硝酸银溶液中24 h，经显影固定脱矿后，切成90 nm超薄切片，铀、铅染色后于透射电镜下观察银离子的渗入情况及界面相关结构。结果4种粘接剂形成的混合层及粘接层薄厚不同：粘接剂A、C混合层较B、D厚，粘接层较B、D簿。4种粘接剂粘接界面均有不同程度纳米渗漏：A(45.02 ±9.49)；B(43.97±8.55)；C(27.02±10.86)；D(12.94±2.07)。粘接剂D组纳米渗漏较小，与其他3种粘接剂间差异有统计学意义(P＜0.05)。结论4种自酸蚀粘接剂混合层和粘接层厚度存在差异，粘接界面的纳米渗漏率不同，一步法自酸蚀粘接系统纳米渗漏较两步法系统明显。

abstractsObjective To evaluate the nanoleakage and ultramorphology of four self-etching adhesives. Methods Sixteen freshly extracted, caries-free human third molars were selected. A flat dentin surface was exposed by removing occlusal enamel. All teeth were randomly divided into four groups acorrding to four different self-etch adhesive: Adper Prompt( A), iBond(B) , Xeno Ⅲ (C) and SE Bond (D). The dentin were bonded with dentin adhesive system according to manufacturer's directions. Composite layers were built up incrementally. The specimens were sectioned longitudinally across the resin-dentin interface into 4. 0 mm × 0. 9 mm sticks and then traced with ammoniacal silver .solution. Epoxy resin-embedded sections were prepared for transmission electron microscope( TEM ) to observe nanoleakage. The images were qualitatively compared by NIH software, and data was analyzed by SPSS. Results Different thickness of hybrid layer and adhesives layer were observed for each adhesive. The hybrid layer of A, C was thicker than that of B, D, and adhesive layer of D was thicker than the others. The extent of nanoleakage varied among different adhesives: A (45.02 ±9.49),B (43.97 ±8.55), C(27.02 ± 10.86), D (12.94 ±2.07). D presented significantly less silver deposition than any of the others did (P ＜ 0. 05 ). Conclusions The thickness of hybrid layer and adhesive layer vary among the four adhesives. The shape and extent of nanoleakage of each adhesive are also different. Two-step system shows less nanoleakage than one-step systems do.