摘要目的 评价骨形态生成蛋白2( bone morphogenetic protein-2,BMP-2)基因强化骨髓间充质干细胞(bone marrow stromal cells,BMSC)复合Bio-Oss骨在上颌窦底提升术中的效果.方法 抽取12只犬髂部骨髓体外分离扩增BMSC,分别加入含BMP-2基因、绿色荧光蛋白(green fluorescent protein,GFP)基因的腺病毒载体共培养,观察转染效率,并植入裸鼠皮下观察成骨情况.将BMP-2基因强化BMSC-Bio-Oss复合骨随机植入犬一侧上颌窦底提升形成的空腔中为实验组,另一侧植入BMSC-Bio-Oss复合骨为空白对照组.分别于术后30、60、120 d行大体观察、CT检查、组织学检查和新生骨量分析.结果 裸鼠皮下可见新生骨,荧光显微镜示踪可见BMSC.犬上颌窦底提升30 d后,大体观察和CT检查示少量新骨形成,120 d后上颌窦内新骨生成致密,仅有少量Bio-Oss骨.新生骨量分析显示,与空白对照组[(19.67 ±5.73) mm2]相比,实验组新生骨量[(24.74±6.33) mm2]显著增多(P＜0.05).结论 BMP-2基因强化BMSC-Bio-Oss复合骨应用于上颌窦底提升术可获得良好的成骨效果.

abstractsObjective To evaluate the effect of maxillary sinus elevation with gene-enhanced tissue engineering bone in dogs.Methods bone marrow stromal cells (BMSC) derived from dog marrow were cultured,and transduced with the adenovirus carrying bone morphogenetic protein-2(BMP-2) gene( AdBMP-2),the adenovirus carrying green fluorescent protein (GFP) gene (AdGFP) in vitro.The bone formation ability of gene modified BMSC with scaffold was examined in nude mice and in elevated maxillary sinus of dog. Student's t-test was used for statistical analysis with SPSS 11.0 software package. Results Gene transfection efficiency reached up to (83.95 ± 2.43 ) ％ as demonstrated by GFP expression.Ectopic bone formation was detected in nude mice.As for maxillary sinus floor elevation in a dog model,new bone formation area in the AdBMP-2 gene transduced BMSC with Bio-Oss group was significantly higher than in BMSC with Bio-Oss group at 120 d (P ＜ 0.05 ).Conclusions AdBMP-2 gene transduced BMSC can stimulate ectopic bone formation in nude mice,and promote bone formation and maturation in the dog maxillary sinus.