摘要目的 研究盐酸小檗碱对体外培养的人牙周膜细胞(periodontal ligament cells,PDLC)分泌单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)的影响,旨在为利用天然药物治疗牙周病提供依据.方法 从离体牙分离牙周膜组织,体外培养人PDLC.经免疫组化鉴定后的人PDLC首先根据细胞培养液中是否含有10 mg/L脂多糖(lipopolysaccharide,LPS)分为LPS组和无LPS组(NLPS组),然后两组再依据培养液中盐酸小檗碱的终质量浓度(0、0.01、0.02、0.03 g/L)分为LPS空白对照组、LPS 1、2、3组及NLPS空白对照组和NLPS 1、2、3组.应用酶联免疫吸附测定法分别检测各组细胞培养上清液中MCP-1的含量,对多组间结果比较采用方差分析,组间两两比较采用Bonferroni t检验,以双侧P＜0.05为差异有统计学意义.结果 NLPS 1、2、3组MCP-1含量[分别为(11.33±0.16)、(11.45 ±0.53)、(11.25 ±0.14) ng/L]与NLPS空白对照组[(11.32±0.35) ng/L]相比差异均无统计学意义(P值均大于0.05)；LPS 1、2、3组MCP-1含量[分别为(38.14±5.34)、(34.15 ±3.36)、(26.13 ±2.12) ng/L]与LPS空白对照组[(58.42±1.52) ng/L]相比均显著降低,差异均有统计学意义(P值均小于0.001)；LPS 1、2、3组间两两比较差异均有统计学意义(P值均小于0.001).结论 当以LPS作为刺激因子时,盐酸小檗碱抑制体外培养的PDLC分泌MCP-1作用明显,并在一定范围内呈浓度依赖性.

abstractsObjective To observe the effects of berberine hydrochloride on the secretion of monocyte chemoattractant protein-1 ( MCP-1 ) from human periodontal ligament cells(PDLC) in vitro culture.Methods Periodontal ligament was isolated from extracted human premolars,and PDLC were cultured in vitro.PDLC were divided into two groups,lipopolysaccharide (LPS) group and non-lipopolysaccharide (NLPS) group.Then in accordance with the final concentrations of berberine hydrochloride in cells culture medium(0,0.01,0.02,0.03 g/L),the groups were subdivided into LPS and NLPS control group,LPS1 and NLPS1 group,LPS2 and NLPS2 group,LPS3 and NLPS3 group.Cellular concentration of MCP-1 of each group was determined by enzyme-linked immunosorbent assay(ELISA).The data were statistically analyzed.Results The MCP-1 contents were not significantly different between the groups of NLPS1,NLPS2 and NLPS3 [ ( 11.33 ±0.16 ),( 11.45 ± 0.53 ),( 11.25 ± 0.14 ) ng/L,respectively ]and the NLPS control group[ ( 11.32 ± 0.35 ) ng/L] ( P =0.692,0.568,0.524).MCP-1 contents in the groups of LPS1,LPS2 and LPS3 [ respectively ( 38.14 ± 5.34 ),( 34.15 ± 3.36 ),(26.13 ± 2.12 ) ng/L ] were significantly lower than in LPS control group [(58.42 ± 1.52) ng/L],P =0.000,0.000,P =0.000. Conclusions The inhibitory effect of berberine hydrochloride on the activities of MCP-1 from PDLC was more significant when PDLC were stimulated with LPS and in a concentration-dependent manner.