摘要目的 探讨存活蛋白(survivin)与口腔鳞状细胞癌发生和预后的相关性.方法 收集口腔鳞状细胞癌21例(试验组)及良性肿物10例(对照组)的口腔脱落细胞、唾液、血清及组织,应用酶联免疫吸附测定和实时定量聚合酶链式反应技术检测标本中存活蛋白及mRNA表达.结果 试验组脱落细胞的存活蛋白表达质量浓度[(43.93±33.30) ng/L]显著高于对照组[(8.49±3.73)ng/L] (P＜0.01)；口腔脱落细胞存活蛋白表达浓度随淋巴结转移而升高(P＜0.05)；口腔鳞状细胞癌组织存活蛋白mRNA的表达通过相对定量倍数关系比较,为正常组织的3.3倍,差异有统计学意义(P＜0.01).结论 存活蛋白在口腔癌的发生过程中起重要作用,口腔脱落细胞的存活蛋白检测可早期诊断口腔鳞状细胞癌并判断预后.

abstractsObjective To investigate the expression of survivin protein and survivin mRNA in oral squamous cell carcinoma (OSCC).Methods A total of 21 patients with OSCC were studied and 10 patients with oral benign disease served as control.Saliva,oral exfoliated cells and serum were collected from the patient before operation.The protein contents of survivin in all samples were quantitatively determined by enzyme-linked immunosorbent assay (ELISA).The expression of survivin mRNA was determined by real-time PCR.Results The protein contents of survivin in exfoliated cells in OSCC(43.93 ±33.30 ng/L)were significantly higher than those in control group (8.49 ± 3.73 ng/L) (P ＜ 0.01).The survivin expression was correlated with lymph nodes metastasis (P ＜ 0.05).The expression of survivin mRNA in OSCC was significantly higher than that in control group (P ＜ 0.01).The expression of survivin mRNA and survivin protein did not correlate with gender,location,differentiation and TNM staging of tumor (P ＞ 0.05).Conclusions Survivin may play a role in the carcinogenesis of OSCC.Detection of survivin in oral exfoliated cells may be a non-invasive method in the diagnosis of OSCC.