摘要目的 评价甲基丙烯酰氧乙基磷酸胆碱(2-methacryloyloxyethyl phosphorylcholine, MPC)和无定形纳米磷酸钙颗粒(nanoparticles of amorphous calcium phosphate,NACP)改性牙本质粘接剂的抗蛋白附着和细菌黏附性能,为预防继发龋提供新思路.方法 以商品化的牙本质粘接剂(Scotchbond Multi-Purpose)作为对照组,在商品化牙本质粘接剂中添加质量分数为7.5％的MPC和20％的NACP作为改性组.制作牙本质粘接试件(每组10个),测试抗剪切强度,即牙本质粘接强度.制作粘接剂试件(每组26个),双辛丁酸法评价抗蛋白附着性能;用人唾液培养所得的牙菌斑全菌生物膜模型研究附着于粘接剂试件表面的牙菌斑全菌生物膜的乳酸产量和活/死菌染色情况.结果 对照组和改性组牙本质粘接强度分别为(29.5±2.9)和(28.7±2.2) MPa,差异无统计学意义(P=0.593).改性组蛋白附着量[(0.21±0.02) μg/cm2]显著低于对照组[(4.17±0.45) μg/cm2] (P=0.000).改性组表面菌斑的乳酸产量[(7.71±1.07) mmol/L]显著低于对照组[(19.18±2.34) mmol/L](P=0.000).活/死菌染色显示改性组试件表面黏附的活细菌量明显少于对照组.结论 本项研究用MPC和NACP改性的牙本质粘接剂,改性后粘接强度无改变,而蛋白附着量和细菌黏附显著降低.

abstractsObjective To evaluate the effect of 2-methacryloyloxyethyl phosphorylcholine(MPC) and nanoparticles of amorphous calcium phosphate(NACP) on the protein-repellent property of dental adhesive.Methods MPC and NACP were incorporated into SBMP as the test group.Scotchbond Multi-Purpose(SBMP) was used as control group.Human dentin shear bond strengths were measured.Protein adsorption onto samples was determined by micro bicinchoninic acid(BCA) method.A dental plaque microcosm biofilm model with human saliva as inoculum was used to investigate biofilm viability.Results The dentin bond strength of modified group was (28.7±2.2) MPa,which was not significantly different from that of the SBMP control group.The amount of protein adsorption in the modified group and the SBMP control group were (0.21 ±0.02) μg/cm2 and (4.17±0.45) μg/cm2 respectively.Lactic acid production of biofilms in modified group and SBMP control were (7.71 ± 1.01) mmol/L and (19.18 ± 2.34) mmol/L repectively.Conclusions MPC-NACP based dental adhesive greatly reduce the protein adsorption and bacterial adhesion,without compromising dentin shear bond strength.This novel bonding agent may have wide application.