摘要目的 探讨肝细胞癌上调的长链非编码RNA(long non-coding RNA highly upregulated in liver cancer,lncRNA HULC)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)细胞系生物学行为的影响.方法 纳入2017年3月至2018年3月于北京大学深圳医院就诊的OSCC患者30例,在肿瘤扩大切除术中提取OSCC和癌旁组织.使用实时荧光定量PCR检测lncRNA HULC在OSCC及相应癌旁组织和OSCC细胞系中的表达水平.用siRNA转染SCC15和SCC25细胞,通过细胞计数实验、划痕实验、Transwell实验和蛋白质印迹法检测lnRNA HULC对OSCC细胞生物学行为的影响.结果lncRNA HULC在OSCC组织中的表达(10.98±0.31)显著高于癌旁组织(8.39±0.31)(t=5.93,P<0.001),在OSCC细胞系中的表达(SCC15:28.58±2.74;SCC25:16.56±0.87;SCC9:11.18±1.32;CAL27:13.92±0.99)均显著高于人类角质形成细胞(1.01±0.00)(P<0.001).SCC15和SCC25细胞中lncRNA HULC表达下调可抑制肿瘤细胞的增殖、迁移和侵袭能力,促进肿瘤细胞的凋亡.结论 lncRNA HULC在OSCC中高表达,且可增强癌细胞的增殖、迁移和侵袭能力,抑制肿瘤细胞凋亡.

abstractsObjective To investigate the effect of long non-coding RNA highly upregulated in liver cancer (lncRNA HULC) on the biological behavior of oral squamous cell carcinoma (OSCC) cell lines. Methods A total of thirty patients with oral squamous cell carcinoma at Peking University Shenzhen Hospital from March 2017 to March 2018 were enrolled in this study. OSCC and adjacent tissues were extracted during tumor extensive resection. Quantitative real-time PCR (qPCR) was used to detect the expression levels of lncRNA HULC in OSCC and paracancerous tissues and OSCC cell lines. SCC15 and SCC25 cells were transfected with siRNA, and the effects of the gene on the biological behavior of OSCC cells were detected by cell counting assay, scratch assay, Transwell assay and Western blotting. Results The expression of lncRNA HULC in OSCC tissues (10.98 ± 0.31, n=30) was significantly higher than that in paracancerous tissues (8.39±0.31, n=30) (t=5.93, P<0.001), the expression of lncRNA HULC in OSCC cells (SCC15:28.58 ± 2.74;SCC25:16.56 ± 0.87;SCC9:11.18 ± 1.32;CAL27:13.92 ± 0.99, n=5) was significantly higher than that in human keratinocytes (1.01±0.00, n=5) (tSCC15=10.08, tSCC25=17.96, tSCC9=7.71, tCAL27=13.09, P<0.001). Down-regulation of lncRNA HULC in SCC15 and SCC25 cells can inhibit the proliferation, migration and invasion of tumor cells and promote tumor cell apoptosis. Conclusions lncRNA HULC is highly expressed in OSCC and can enhance the proliferation, migration and invasion of cancer cells and inhibit tumor cell apoptosis.