摘要目的 了解温州地区宫颈癌患者人乳头状瘤病毒(human papillomavirus,HPV)的感染现状及危险因素,为HPV防治提供流行病学依据.方法 采用导流杂交基因分型技术(HybriMax)对温州地区198例宫颈癌患者的宫颈脱落细胞样本进行HPV检测及基因分型,分析HPV感染与宫颈癌临床分期、病理类型及分化程度的关系,并对不同宫颈疾病患者的HPV感染情况进行比较.采用SPSS 13.0软件进行统计分析.结果 198例宫颈癌患者中,HPV感染147例,占74.24%,其中重叠感染101例(51.01%),高危型HPV感染129例(65.15%),均高于宫颈炎和宫颈不典型增生患者,3组比较差异有统计学意义(x2值分别为28.28、65.34和95.22,P值均＜0.01).宫颈癌患者中,不同临床分期患者HPV感染率比较差异无统计学意义(x2=0.475,P＞0.05);鳞癌与腺癌患者的HPV感染率比较差异无统计学意义(x2=0.582,P＞0.05);不同分化程度患者的HPV感染率比较差异亦无统计学意义(x2=0.969,P＞0.05).Logistic多元回归分析显示,HPV16/58型感染和年龄＞40岁是宫颈癌发生的主要危险因素.结论 温州地区宫颈癌患者生殖道有较高的HPV感染率及重叠感染率,HPV16/58感染和40岁以上妇女发生宫颈癌的危险增加,故应加强对该人群的检测并尽早采取干预措施.

abstractsObjective To investigate the prevalence of human papillomavirus(HPV)infections in women with uterine cervical cancer in Wenzhou.Methods Exfoliated cells samples of cervix uteri were collected from 198 patients with cervical cancer. Flow-through hybridization technique was used to detect HPV and its genotypes.The relationship of HPV infection with cervical cancer stage,histological type and differentiation degree were analyzed.The prevalence of HPV infections in patients with different cervical diseases was observed.SPSS 13.0 was used for statistical analysis.Results In 198 patients with cervical cancer,HPV infection was occunrred in 147 (74.24%), of whom 101patients were superinfected (51.01%),and 129 patients(65.15%)were infected with the high-risk HPVs,which were significantly higher than those in cervicitis and cervical dysplasia(x2 = 28.28,65.34 and 95.22,P ＜ 0.01).HPV positive rate was not correlated with clinical stages,differentiation degree of cervical cancer(x2 = 0.475 and 0.969,P＞0.05).HPV positive rates in squamous cancer and adenocarcinoma had no statistical difference (x2 =0.582,P＞0.05).The logistic regression analysis showed that HPV 16/58 infection and age over 40might increase the risk of carcinogenesis of the cervix.Conclusion sHPV infection and superinfection are popular in women with cervix cancer in Wenzhou.HPV16/58 infection and age over 40 years are the risk factors of cervical cancer.