摘要目的 探讨乙型肝炎肝纤维化患者肠道微生态特点及血清代谢谱的改变.方法 选择2011年1月至2012年12月就诊于浙江省金华市中心医院的30例肝纤维化患者和100名健康人作为研究对象.采集所有研究对象的新鲜粪便和血液样本各1份.运用聚合酶链反应(PCR)和变性梯度凝胶电泳(DGGE)技术对粪便样本进行检测并生成16S rDNA V3可变区基因的DGGE图谱,然后用Matlab 7.0软件进行主成分分析,以寻找两组肠道微生态图谱差异.血液样本采用1H磁共振技术检测与分析两组血清代谢物图谱的差异.结果 对粪便样本进行PCR-DGGE检测和主成分分析,结果显示,与健康人群相比,乙型肝炎肝纤维化患者肠道主要菌群结构发生了明显变化.血清代谢图谱分析显示:肝纤维化组和健康对照组的血清代谢谱完全不同,肝纤维化患者血清代谢物中乳酸、蛋氨酸、三甲胺和葡萄糖明显升高.结论 肝纤维化患者肠道总菌群结构及代谢产物与健康人之间有明显差异.

abstractsObjective To investigate the characteristics of intestinal microflora and the changes of serum metabolite profile in patients with hepatitis B-related liver fibrosis.Methods Fecal and blood samples were collected from 30 patients with hepatitis B-related liver fibrosis and 100 healthy controls in Jinhua Central Hospital during January 2011 and December 2012.Polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) were performed in fecal samples to get 16S rDNA V3-DGGE profiles.And principal component analysis was performed using Matlab 7.0 software to explore the difference in intestinal microflora between patients and healthy controls.1 H nuclear magnetic resonance technique was used to reveal the difference in serum metabolite profiles between two groups.Results DGGE profiles and principal component analysis showed significant difference in the structure of intestinal microflorae between healthy individuals and patients with hepatitis B-related liver fibrosis.Serum metabolite profiles showed that,compared with the healthy individuals,lactic acid,methionine,trimethylamine and glucose increased significantly in patients with hepatitis B-related liver fibrosis.Conclusion The structure of intestinal microflora and serum metabolite profile in patients with hepatitis B-related liver fibrosis are different from healthy individuals.