摘要目的：观察肝螺杆菌在雄性和雌性BALB／cCr小鼠肝脏的定植状况及肝脏的病理特征。方法选取证实无肝螺杆菌感染的SPF级雌性和雄性BALB／cCr小鼠各25只，口服灌饲肝螺杆菌标准菌株ATCC 51450菌液0．2 mL（1×108CUF／mL），连续3次，每次间隔48 h；对照组（雌、雄各25只）灌饲等量的磷酸盐缓冲液（PBS）。于末次接种肝螺杆菌后第1，3，6，9和12个月时，禁食12 h后处死小鼠，每组5只。应用酶联免疫吸附试验（ ELISA）检测血清肝螺杆菌-IgG抗体水平；取小鼠肝脏组织，行病理组织学检查和微需氧菌分离培养鉴定。采用t检验进行不同时间点和各组间肝螺杆菌-IgG抗体水平和肝脏病理组织学评分比较。结果雄性BALB／cCr小鼠血清肝螺杆菌-IgG抗体均为阳性，在感染后6个月达最高峰，以后逐渐下降，感染后3～12个月时的抗体水平高于感染后1个月（t＝2．828，4．300，3．536和4．500，P＜0．05）；雌性BALB／cCr小鼠在感染后第9、12个月时各有一只小鼠肝螺杆菌-IgG抗体为阳性。雄性BALB／cCr小鼠肝螺杆菌感染后3个月时在肝组织中发现有肝螺杆菌定植，而雌性BALB／cCr小鼠肝组织中未发现肝螺杆菌定植。肝螺杆菌感染的雄性BALB／cCr小鼠各时间点肝脏病理组织学评分均明显高于雌性BALB／cCr小鼠（ t＝2.598，7.770，7.987，10.850和12.260，P＜0.05或P＜0.01），且雄性BALB／cCr小鼠肝螺杆菌感染后6个月内随着感染时间的延长肝脏病理组织学评分逐渐增加（t＝4.949，P＜0.01），6至12个月肝脏病理组织学评分有加重的趋势，但差异无统计学意义（ P＞0.05）。结论雄性BALB／cCr小鼠肝螺杆菌感染后肝脏定植率及病理组织学改变均较雌性BALB／cCr小鼠更为明显，且病理组织学改变随着感染时间的延长而逐渐加重。

abstractsObjective To observe pathological features of liver injury induced by Helicobacter hepaticus ( H.hepaticus) and the difference between male and female BALB/cCr mice.Methods Fifty SPF-class BALB/cCr mice (25 males and 25 females) were administrated by gavage with 0.2 mL bacterial suspension (1 ×108 CUF/ml) of H.hepaticus standard strain ATCC 51450 for 3 times with 48 h intervals. The control group (25 males and 25 females) received same volume of phosphate buffered saline (PBS). Mice were sacrificed in batches ( n=5) after fasting for 12 h at month 1, 3, 6, 9 and 12.Enzyme linked immunosorbent assay ( ELISA) was used to determine the serum level of H.hepaticus IgG antibodies.Liver tissue samples were taken for histopathology examiantion, micro-aerobic bacteria isolation, culture and identification.t test was used to analyze the differences in serum levels of H.hepaticus IgG antibody and liver histopathologic scores between different time points and groups. Results The seroprevalance of H. hepaticus-IgG antibody in male BALB/cCr mice infected with H.hepaticus were all positive, peaked at 6 month, and then gradually declined.H.hepaticus-IgG antibody levels at 3, 6, 9 and 12 month were higher than that at 1 month (t=2.828, 4.300, 3.536 and 4.500, P<0.05).Only one female BALB/cCr mouse infected with H.hepaticus was positive for H.hepaticus-IgG antibody at 9 and 12 month.H.hepaticus&nbsp;colonization in liver was observed in male BALB/cCr mice infected with H.hepaticus since month 3, while it was not observed in female BALB/cCr mice.Compared with femle mice, the histopathologic scores of liver in male mice infected were much higher at all time points (t=2.598,7.770,7.987,10.850 and 12.260, P<0.05 or P<0.01) .Liver histopathologic scores increased in male mice as infection time extended within 6 months (t=4.949, P<0.01), but not during 6 and 12 month (t=1.052, P>0.05).Conclusion Compared with female mice, H.hepaticus colonization and histopathologic changes in liver are more significant in male BALB/cCr mice infected with H.hepaticus, and the histological scores are increased as infection time extended.