摘要目的 探讨纳米氧化铝颗粒致小鼠脑氧化应激损伤效应.方法 选取健康成年雄性ICR小鼠60只,按体重随机分为6组:空白对照组(未做任何处理,同等条件饲养)、溶剂对照组(每日鼻腔滴注生理盐水)、微米氧化铝组(给予100 mg/kg微米氧化铝)及纳米氧化铝低、中、高剂量组(分别为给予50、100、200mg/kg纳米氧化铝),每组10只.不同剂量的纳米氧化铝经鼻腔滴注染毒30 d.测定脑组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力.结果 与空白对照组[(17.32±6.23)U/gHb]相比,纳米氧化铝低剂量组小鼠脑组织中SOD活力[(17.89±1.82)U/gHb]有增加的趋势,但差异无统计学意义(P>0.05);纳米氧化铝中、高剂量组小鼠SOD活力[分别为(14.23±2.21)、(4.93±2.30)U/gHb]下降,但只有高剂量组的差异有统计学意义(P<0.05).与空白对照组[(0.24±0.09)nmol/ml]相比,纳米氧化铝低、中、高剂量组小鼠脑组织中MDA含量[分别为(0.76±0.13)、(1.00±0.30)、(1.16±0.39)nmol]ml]均明显增加,差异均有统计学意义(P<0.05).与空白对照组[(1.55±0.34)mg/gpro]相比,纳米氧化铝低、中、高剂量组小鼠脑组织中GSH含量[分别为(0.72±0.08)、(0.55±0.19)、(0.61±0.20)mg/gpro]均明显降低,差异均有统计学意义(P<0.05).但各组小鼠GSH-Px活力间的差异无统计学意义(P>0.05).与空白对照组[(5.79±0.96)U/mgpro]相比,纳米氧化铝低、中剂量组小鼠脑组织中CAT活力[分别为(10.40±3.84)、(10.40±2.00)U/mgpro]升高,高剂量组[(3.25±1.04)U/mgpro]CAT活力下降差异均有统计学意义(P<0.05).结论 纳米氧化铝可以导致ICR小鼠脑组织的氧化应激损伤.

abstractsObjective To investigate the brain oxidative stress injury induced by nano-alumina particles in ICR mice.Methods Sixty male ICR mice were randomly divided into 6 groups:control group,solvent control group,100 mg/kg micro-alumina particles group,3 groups exposed to nano-alumina particles at the doses of 50,100 and 200 mg/kg.The mice were exposed by nasal drip for 30 days.Then levels of malondialdehyde (MDA),glutathione (GSH),superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase ( GSH-PX ) in brain tissues of mice were detected.Results There was no difference of SOD activity in mouse brain between control group[(17.32 ±6.23)U/gHb]and 50 mg/kg nano-alumina particles group[(17.89±1.82) U/gHb].The SOD activity[(4.93±2.30 )U/gHb]in 200 mg/kg nano-alumina particles group was significantly lower than that in control group (P<0.05 ).The MDA levels in 3 nano-alumina particles groups were (0.76±0.13),(l.00±0.30) and (1.16±0.39)nmol/ml,respectively,which were significantly higher than that[(0.24±0.09)nmol/ml]in control group (P<0.05 ).The GSH levels in 3 nano-alumina particles groups were (0(0.72±0.08),(0.55±0.19) and (0.61 ±0.20)mg/gpro,respectively,which were significantly lower than that[(1.55±0.34)mg/gpro]]in control group (P<0.05 ).The CAT activity in 50 and 100 mg/kgnano-alumina particles groups were (10.40±3.84) and (10.40±2.00)U/mgpro,respectively,which were significantly higher than that[(5.79±0.96) U/mgpro]in control group (P<0.05 ).The CAT activity[(3.25±1.04)U/mgpro]in 200 mg/kg nano-alumina particles group was significantly lower than that in control group (P<0.05 ).Conclusion Nano-alumina particles can induce the oxidative press damage in brain tissues of mice.