摘要目的 研究温石棉(CA)与岩棉(RW)和硅灰石(WS)纤维的细胞毒性.方法 将V79细胞分为CA组、RW组及WS组3个实验及1个阴性对照组(200 μl PBS),CA、RW及WS粉尘终质量浓度为100 mg/L,噻唑蓝(MTT)法观察V79细胞存活率,用动力学法测乳酸脱氢酶(LDH)活力,用扫描电子显微镜观察粉尘对V79细胞形态的影响.结果 SiO2均为3种纤维的主要成分,WS中的SiO2含量最高,为50.83%.CA组、RW组、WS的细胞存活率分别为54.5%、64.8%、65.7%,RW组和WS 组的存活率明[显高于CA组,差异有统计学意义(P＜0.01),RW组和WS组的LDH活力[分别为(15.7±0.9)、(12.3±3.7)U/L]明显低于CA组[(20.2±0.9)U/L].差异有统计学意义(P＜0.05).CA组的V79细胞两端堆积大量颗粒状残余体,RW组和WS组V79细胞形态基本正常.结论 RW和WS对V79细胞的毒性低于CA.

abstractsObjective To study the cytotoxicity induced by chrysotile asbestos (CA), rock wool (RW)and wollastonite (WS). Methods V79 cells were divided into 4 groups. i.e. CA group, WS group, RW group and control group (200 μ1 PBS). The exposure concentration of dusts was 100 mg/L, The cell viability was detected by MTT and lactate dehydrogenate (LDH) activity assays. The technique of scanning electron microscopy was used to examine the change of V79 cells. Results SiO2 was main constituent for 3 kinds of dusts. In MTT assay, the cell viability of RW and WS groups was 64.8% and 65.7%, respectively, which were significantly higher than that (54.5%) of CA group (P＜0.01). In LDH assay, the LDH activity of RW and WS groups [(15.7±50.9),( 12.3±3.7)U/L, respectively] was significantly lower than that[( 20.2±0.9) U/L]of CA group (P＜0.05). In scanning electron microscopy examination, it was found that the two ends of V79 cells in CA group contained a great deal of fibers remaining bodies, but the V79 cell appearance in RW and WS groups was normal. Conclusion The cytotoxicity induced by RW and WS is significantly lower than that induced by CA for V79 cell.