摘要目的 研究吸烟对焦炉作业工人尿中多环芳烃(PAHs)代谢物浓度的影响.方法 采用高效液相色谱法检测工人工作环境中颗粒物载带上的PAHs浓度；通过职业健康体检和问卷调查的方式收集某焦化厂1401名男性焦炉工人的职业信息,并采集尿液；使用气相色谱-质谱联用仪测定尿样中10种羟基多环芳烃代谢产物(OH-PAHs)的浓度.按照工作岗位和工作环境PAHs浓度的差异分成对照组、低暴露组、中暴露组和高暴露组4组,采用协方差分析分别比较各组吸烟者与不吸烟者尿中10种OH-PAHs的浓度差异.结果 工作环境中16种PAHs的检测结果均显示高暴露组＞中暴露组＞低暴露组＞对照组,高暴露组的浓度明显高于对照组、低暴露组和中暴露组,差异有统计学意义(P＜0.05).在对照组、低暴露组、中暴露组和高暴露组中,吸烟者尿中1-羟基萘(1-OHNa)和2-羟基萘(2-OHNa)的浓度明显高于不吸烟者,差异有统计学意义(P＜0.05)；4个组中,吸烟者的1-羟基芘(1-OHP)浓度与不吸烟者的差异无统计学差异(P＞0.05).结论 在焦炉作业人群中,尿中1-OHNa和2-OHNa可以作为评价吸烟对工人尿中OH-PAHs浓度影响的生物标志物.

abstractsObjective To explore the effects of smoking on urinary 10 metabolites of polycyclic aromatic hydrocarbons (PAHs) in the coke oven workers.Methods Occupational health examination was performed on 1401 coke oven workers in one coking plant,their urine were collected respectively.The concentrations of the ten monohydroxy polycyclic aromatic hydrocarbons in urine were detected by gas chromatography/mass spectrometry.The 1401 workers were divided into four groups,namely control,adjunct workplaces,bottom and side,top group according to their workplaces and the different concentrations of PAHs in the environment.The concentrations of the ten monohydroxy polycyclic aromatic hydrocarbons between smokers and nonsmokers in each workplace group were compared using analysis of covirance,respectively.Results The levels of concentrations of the sixteen polycyclic aromatic hydrocarbons we detected at control were significantly higher than those at other areas (P＜0.05).Comparing the ten monohydroxy polycyclic aromatic hydrocarbons levels between smokers and nonsmokers,the levels of l-hydroxynathalene and 2-hydroxynathalene among smokers were higher than nonsmokers with statistically significance in control,adjunct workplaces,bottom and side and top groups(P＜0.05).However,the levels of 1-hydroxypyrene had no statistically significant differences between the four areas.Conclusion Urinary 1-hydroxynathalene and 2-hydroxynathalene may be used as biomarkers for the impact of smoking on monohydroxy polycyclic aromatic hydrocarbons in the coke oven workers.