摘要目的 探讨焦化厂工人ERCC6基因多态性和外周血淋巴细胞DNA损伤的关联.方法 通过整群抽样的方法选择焦化厂工作8h班后焦炉工379名作为暴露组,焦化厂班后休息16h以上的398名焦炉工作为恢复组,同时选择同厂非多环芳烃(PAHs)职业暴露者398名工人为对照组,采集外周静脉血,分离淋巴细胞,用单细胞凝胶电泳试验评价DNA损伤;用TaqMan-MGB探针检测ERCC6基因分型;运用PHASE2.0.2遗传分析软件计算单体型.结果 暴露组工人外周血淋巴细胞的Olive尾矩(OTM)(-0.86±0.70)高于对照组(-1.13±0.65)和恢复组(-1.14±0.68),差异有统计学意义(P＜0.05).暴露组按工人年龄分层后,年龄≥37岁组ERCC6基因rs3793784位点CG+GG基因型携带者的外周血淋巴细胞的OTM值明显低于CC基因型携带者,年龄＜37岁组ERCC6基因rs3793784位点CC基因型携带者外周血淋巴细胞OTM值明显低于年龄≥37岁的CC基因型携带者,年龄＜37岁的组ERCC6基因rs3793784位点GG+GC基因型携带者外周血淋巴细胞OTM值明显高于年龄≥37岁的GG+GC基因型携带者,差异均有统计学意义(P＜0.05).按内暴露分层,在1-羟基芘＞4.36μmol/L组的AG+GG基因型携带者外周血淋巴细胞OTM值明显高于AA型携带者,差异有统计学意义(P＜0.05).结论 焦炉工外周血淋巴细胞ERCC6基因rs3793784在暴露组中不同的基因型在不同的年龄段存在不同的作用,提示了PAHs暴露人群中基因型和年龄可能存在交互作用.

abstractsObjective To investigate the association between ERCC6 gene polymorphisms and peripheral blood lymphocyte DNA damage among the workers in coking plant.Methods By cluster sampling,379 coke oven workers having worked for 8 hours were included in the exposure group,398 coke oven workers having rested for more than 16 hours were included in the recovery group,and 398 workers having never been exposed to polycyclic aromatic hydrocarbons (PAHs) in the same plant were included in the control group.Lymphocytes were separated from their peripheral venous blood,and single cell gel electrophoresis was used to evaluate DNA damage; TaqMan-MGB probes were used to analyze ERCC6 gene polymorphisms.PHASE 2.0.2 genetic analysis software was used to calculate the haplotypes.Results The Olive tail moment (OTM) of lymphocytes in the exposure group was significantly higher than those in the recovery group and control group (-0.86±0.70 vs-1.14±0.68 and-1.13±0.65,P＜0.05).In the exposure group,for workers ≥37 years old,the OTM of lymphocytes in workers carrying CG+GG genotype at rs3793784 locus of ERCC6 gene was significantly lower than that in workers carrying CC genotype (P＜0.05); the OTM oflymphocytes in workers ＜37 years old carrying CC genotype at rs3793784 locus of ERCC6 gene was significantly lower than that in workers ≥ 37 years old carrying CC genotype (P＜0.05); the OTM of lymphocytes in workers ＜37 years old carrying CG+GG genotype at rs3793784 locus of ERCC6 gene was significantly higher than that in workers ≥37 years old carrying CG+GG genotype (P＜0.05).For patients with internal exposure,in the 1-hydroxypyrene ＞4.36 μmol/L group,the OTM of lymphocytes in workers carrying AG+GG genotype was significantly higher than that in workers carrying AA genotype (P＜0.05).Conclusion Different genotypes of ERCC6 gene rs3793784 in peripheral blood lymphocytes of coke oven workers exposed to PAHs have different functions at different ages,suggesting that genotype may interact with age in population exposed to PAHs.