摘要目的 建立电感耦合等离子体质谱(ICP-MS)同时检测血中总铅浓度和铅同位素的方法.方法 肝素抗凝全血用5％硝酸脱蛋白处理后,在线加入铊(TI)作为内标物,利用冻干牛血铅、镉标准物质(GBW09139h和GBW09140h)对血铅浓度进行质量控制.利用铅同位素标准物质(GBW04426)确定铅同位素比值校正系数,将铅同位素标准参考物NIST SRM981同样品同时用ICP-MS检测.结果 对检测方法进行优化后,检测血铅及铅同位素,该方法线性范围广(r ＞0.9999),GBW09139h和GBW09140h检测结果均在质控范围内.同位素测定精密度高,NIST SRM981相对标准偏差＜1％,NISTSRM981检测结果与证书值接近.结论 该方法方便简捷、数据可靠,可满足血中总铅和前同司位素的同时测定.

abstractsObjective To establish a method to determine lead and Isotope Ratios in whole blood by inductively coupled plasma mass spectrometry (ICP-MS).Methods The whole blood samples was removed protein by 5％ nitric acid,Online join thallium (Tl) as internal standard substance,used lyophilized bovine blood lead and cadmium standard material (GBW09139h and GBW09140h) for quality control of blood lead concentrations.Lead isotope standard substances (GBW04426) is used to determine the correction factor,lead isotope ratios will lead isotope standard reference material NIST SRM981 by icp-ms with samples for testing.Results Optimize the detection method,detection of blood lead and lead isotope,and the method of linear range r ＞0.9999,GBW09139h and GBW09140h test results are within the scope of quality control.NIST SRM981 isotope determination precision RSD＜1％,NIST SRM981 test results and the certificate of value close to.Conclusion The method is simple and convenient data is reliable,can meet the total lead (pb) in blood and former isotope simultaneous determination.