摘要目的 研究镍钢作业工人hOGG1对8-OH-dG的切除修复能力和镍钢作业工人DNA氧化损伤水平.方法 选取某不锈钢冶炼企业镍作业工人231人,作为镍接触组,该企业水处理厂水泵工75人作为对照组,对工作场所职业性危害因素进行检测;Elisa双抗夹心法测定尿样中8-OH-dG含量,RT-PCR检测修复酶基因hOGG1的mRNA的表达,Pearson相关分析分析尿中8-OH-dG含量和修复酶基因hOGG1的mRNA表达水平的相关性.结果 镍接触组不同工种8-OH-dG含量与对照组比较,轧钢工组差异无统计学意义(P＞0.05),炼钢工组、钢渣处理工组与对照组比较的差异均有统计学意义(P＜0.05);镍接触组不同工龄8-OH-dG含量与对照组相比,工龄0～5年组和6～10年组与对照组差异无统计学意义(P＞0.05),其他各组与对照组的差异均有统计学意义(P＜0.05).不同工种hOGG1 mRNA表达水平与对照组相比,差异均有统计学意义(P＜0.05);不同工龄组hOGG1mRNA表达水平与对照组比较,工龄0～5年组与对照组差异无统计学意义(P＞0.05),其他各组差异均有统计学意义(P＜0.05).Pearson分析不同工种工人尿中8-OH-dG含量与hOGG1mRNA表达水平之间的相关性,r=0.993,呈正相关,在α=0.01(P＜0.05)水平上显著相关;不同工龄工人尿中8-OH-dG含量与hOGG1mRNA表达水平之间的相关性分析,r=0.968,呈正相关,在α=0.01 (P＜0.05)水平上显著相关.结论 镍钢作业工人DNA氧化损伤水平增加,hOGG1基因对8-OH-dG有主动切除修复能力.

abstractsObjective To study the excision repair capacity of human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) for 8-OH-dG and the oxidative DNA damage among workers exposed to nickel in stainless steel production environment.Methods A total of 231 workers exposed to nickel in a stainless steel production enterprise were recruited as nickel exposure group,and another 75 water pump workers in that enterprise were recruited as control group.The workplace occupational hazard factors were determined.Double-antigen sandwich ELISA was used to determine urinary 8-OH-dG level; RT-PCR was used to determine hOGG1 mRNA level.Pearson correlation was used to analyze the correlation between urinary 8-OH-dG level and hOGG1 mRNA level.Results Level of 8-OH-dG was compared between different types of nickel-exposed workers and control workers; rolling mill workers showed no significant difference from the control group (P＞0.05),while steelmaking workers and steel slag disposing workers showed significant differences from the control group (P＜ 0.05).Level of 8-OH-dG was also compared between nickel-exposed workers with different working years and control workers; nickel-exposed workers with 0～5 and 6～10 working years showed no significant differences from the control group (P＞0.05),while other exposed workers showed significant differences from the control group (P＜0.05).Different types of nickel-exposed workers all showed significant differences from the control group in hOGG 1 mRNA level (P＜0.05).Nickel-exposed workers with 0～5 working years showed no significant difference from the control group in hOGG 1 mRNA level (P＞0.05),while other exposed workers showed significant differences from the control group (P＜0.05).Pearson correlation analysis showed that urinary 8-OH-dG level was positively correlated with hOGG1 mRNA level (r=0.993) in different types of nickel-exposed workers,and the correlation was significant at α=0.01 (P＜0.05); urinary 8-OH-dG level also showed a positive correlation with hOGG1 mRNA level in nickel-exposed workers with different working years (r=0.968),and the correlation was significant at α=0.01 (P＜0.05).Conclusion Exposure to nickel increases oxidative DNA damage among steel workers,and hOGG1 shows active excision repair capacity for 8-OH-dG.