摘要目的 探讨不同浓度三氯化铟(InCl3)对人正常肺上皮(Beas-2B)细胞增殖的影响及其可能机制.方法 不同浓度(0、0.3、1.0、3.0、10.0、30.0、90.0、270.0、810.0 μmol/L)InCl3分别处理Beas-2B细胞24、48和72 h,用CCK-8法检测InCl3对细胞增殖的影响;用Annexin V-PI双染,经流式细胞术检测InCl3对细胞凋亡的影响;以2’,7’-二氯荧光黄双乙酸盐为探针,用流式细胞术检测InCl3染毒后Beas-2B细胞内的活性氧(ROS)水平.结果 0.3～3.0 μmol/LInCl3组的细胞存活率明显升高,而30.0～810.0μmol/L InCl3组72 h后细胞存活率降低,与对照组相比,差异有统计学意义(P＜0.05);0.3 μmol/L InCl3组在72 h内都未能引起细胞凋亡的发生,而30.0和810.0 μmol/L InCl3组72 h后引起明显的细胞早期凋亡,0.3 μmol/L InCl3组72h内ROS含量与对照组相比略微下降,而30.0和810.0 μmol/L InCl3组72h后,细胞内ROS含量明显增加,差异有统计学意义(P＜0.05).结论 低浓度InCl3可能通过降低细胞内ROS促进细胞增殖,而高浓度InCl3则通过引起细胞内ROS升高、细胞凋亡而发挥其抑制细胞活力的作用.

abstractsObjective To investigate the effects of different concentrations of indium chloride (InCl3)on the proliferation of human lung epithelial (Beas-2B) cells and its potential mechanism.Methods Beas-2B cells were exposed to different concentrations of InCl3 (0.3,1.0,3.0,10.0,30.0,90.0,270.0,and 810.0 μmol/L)for 24,48,and 72 h,respectively.The effects of InCl3 on cell proliferation were determined by the CCK-8 assay.The effects of InCl3 on apoptosis were evaluated using annexin V-PI staining followed by flow cytometry.The level of intracellular reactive oxygen species (ROS) in Beas-2B cells after exposure to InCl3 was determined using 2′,7′-dichlorofluorescein diacetate labeling followed by flow cytometry.Results Compared with the control group,InCl3 at a relatively low concentration (0.3～3.0 μmol/L) significantly promoted cell proliferation (P＜0.05),while InCl3 at a relatively high concentration (30.0～80.0 μmol/L) significantly inhibited cell proliferation after 72 h (P＜0.05).InCl3 at a concentration of 0.3 μmol/L failed to induce apoptosis within 72 h; however,InCl3 at a concentration of 30.0 or 810.0 μmol/L induced substantial early apoptosis after 72 h.Compared with the control group,cells exposed to 0.3 μmol/L InCl3 showed a slight decrease in the level of intracellular ROS within 72 h,while cells exposed to 30.0 or 810.0 μmol/L InCl3 showed a significant increase in the level of intracellular ROS after 72 h (P＜0.05).Conclusion At a low concentration,InCl3 stimulates cell proliferation by reducing intracellular ROS.However,at a high concentration,InCl3 inhibits cell viability by elevating intracellular ROS and inducing apoptosis.