摘要目的 探讨乙苯对耳蜗前体细胞(CPCs)生长形态、增殖能力及线粒体膜电位(MMP)的影响,为乙苯导致听力损失的机制研究奠定基础.方法 分离SPF级SD新生大鼠原代CPCs,荧光显微镜鉴定分离培养成功后,分为对照组和染毒组经不同浓度(0、15、30、45 μmol/L)的乙苯染毒24 h.采用倒置光学显微镜观察细胞损伤的形态学变化,MTT比色法检测细胞的增殖能力,荧光探针JC-1测定MMP的变化.结果 大鼠CPCs的鉴定结果显示,肌球蛋白(Myosin)VIIa和内吞作用辅助蛋白(Epsin)表达阳性;与对照组相比,各染毒组大鼠CPCs的折光度增强,细胞死亡数目增多;与对照组相比,各染毒组的CPCs增殖能力明显降低并呈一定的剂量-效应关系,差异均有统计学意义(P＜0.05);与对照组相比,各染毒组大鼠MMP明显降低,差异均有统计学意义(P＜0.05).结论 乙苯可能导致大鼠CPCs发生损伤,细胞增殖抑制及MMP降低.

abstractsObjective To investigate the effects of ethylbenzene on growth morphology、proliiferation ability and mitochondrial membrane potential (MMP) of cochlear progenitor cells (CPCs),and to lay a foundation for the mechanism of hearing loss induced by ethylbenzene.Methods We can use the fluorescence microscopy to identify the original CPCs isolated from the newborn rats,and followed by the addition of different concentrations of ethylbenzene (0,15,30,45 μmol/L) for 24 hours.The morphological changes of cell injury were observed by inverted optical microscope.The proliferation ability of cells was detected by MTT colorimetry,and the change of MMP was detected by fluorescent probe JC-1.Results The results of CPCs identification showed the expression of Myosin VIIa and Epsin positive;The results observed by inverted optical microscope showed all groups of CPCs morphological changes compared with the control group;MTT results showed that the decreased significantly proliferation ability of CPCs groups compared with the control group and a dose effect relationship with statistically significant difference (P＜0.05);JC-1 test results showed the decreased significantly mitochondrial membrane potential in the treated group compared with the control group,and there was a statistically significant difference (P＜0.05).Conclusion Ethylbenzene may cause damage to CPCs,inhibition of cell proliferation and decrease of MMP in rats.