摘要目的:探讨二烯丙基一硫（Diallyl sulfide，DAS）对苯诱导大鼠细胞遗传毒性损伤的保护效果。方法:于2018年9月，选取SPF级大鼠60只，适应性喂养5 d后，按体重随机分为6组，对照组、DAS组、苯模型组、苯+DAS低剂量组、苯+DAS中剂量组、苯+DAS高剂量组，每组10只。苯+DAS低中高各剂量组和DAS组分别以40、80、160、160 mg/kg DAS剂量灌胃染毒，对照组和苯模型组给予等体积玉米油。2 h后，苯模型组和苯+DAS各剂量组给予苯（1.3 g/kg）-玉米油混合液（体积分数50%），对照组和DAS组给予等体积玉米油。1次/d，连续4周。收集样品，用于后续指标检测。结果:与对照组比较，苯模型组大鼠外周血白细胞计数（WBC）降低65.06%、淋巴细胞比例降低、微核率（‰）增加（ P=0.003、0.000、0.000），BMCs中γH2AX平均荧光强度和相对荧光强度分别增加32.69%、32.64%（ P=0.001、0.008），PBLs中γH2AX平均荧光强度和相对荧光强度分别增加397.70%、396.26%（ P=0.000、0.003）；与苯模型组比较，苯+DAS低中高各剂量组大鼠WBC计数均增加（ P=0.000、0.003、0.006），微核率（‰）分别降低（ P=0.000、0.000、0.000）；与苯模型组比较，苯+DAS高剂量组BMCs中γH2AX平均荧光强度和相对荧光强度降低明显（ P=0.000、0.000），苯+DAS高剂量组PBLs中γH2AX平均荧光强度和相对荧光强度降低明显（ P=0.000、0.000）。 结论:DAS能有效拮抗苯诱导的大鼠细胞遗传毒性损伤。

abstractsObjective:To investigate the protective effect of diallyl sulfide (DAS) , against benzene-induced genetic damage in rat.Methods:In September 2018, Sixty adult male adaptive feeding 5 days, were randomly divided into six groups according to their weight. Control groups, DAS control groups, benzene model groups, benzene+low DAS groups, benzene+middle DAS groups, benzene+High DAS group, 10 in each group. Rats in the DAS and DAS control group were orally given DAS at 40, 80, 160, 160 mg/kg, blank control and benzene model groups were given corn oil in the same volume. 2 h later, the rats in the benzene model and DAS treatment groups were given gavage administration of benzene (1.3 g/kg) mixed with corn oil (50%, V/V) , blank and DAS control groups were given corn oil in the same volume. Once a day, for 4 weeks. Samples were collected for subsequent testing.Results:Compared with the blank control group, In benzene treated rat, peripheral WBC count was reduced 65.06% ( P=0.003) , lymphocyte ratiowas reduced ( P=0.000) , micronucleus rate was increased ( P=0.000) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in BMCs were increased 32.69%、32.64% ( P=0.001、0.008) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in PBLs were increased 397.70%、396.26% ( P=0.000、 P=0.003) respectively. Compared with the benzene model group, the WBC count increased respectively ( P=0.000、0.003、0.006) and the micronucleus rate decreased ( P=0.000、0.000、0.000) in the DAS groups, Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in BMCs were significantly reduced in the high DAS groups ( P=0.000、0.000) , Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in PBLs were significantly reduced in the low, middle, high DAS groups ( P=0.000、0.000) . Conclusion:DAS can effectively suppress benzene induced genotoxic damage in rats.