摘要目的研究降钙素基因相关肽（CGRP）在不同年龄人群牙髓及牙本质组织中的表达，探讨牙衰老与老年人牙病的临床表现及其机制。方法收集人前磨牙120颗，按年龄分组，石蜡包埋切片，免疫组化染色，图像定量分析，SAS软件统计。结果随年龄增长，CGRP阳性神经束逐渐变细，分支减少，密度降低，伸入前期牙本质的范围缩小；其积分光密度、体密度、线密度、线段长度均减少。各组之间除积分光密度差异无显著性外，其他P值均＜0.01。结论随年龄增长牙髓及牙本质组织中CGRP阳性神经纤维的数量、分支和范围减小，敏感性可能下降。

abstractsObjective　The age-related changes of calcitonin gene-related peptide （CGRP）immunoreactive nerve fibers in dental pulp-dentine complex were studied to explore the clinical signs of dental disease and their mechanism of aging.　Methods　Human premolars were collected and divided into 4 groups according to age. All samples were embedded in paraffin and cut into slices. These slices were treated by immunohistochemical reaction of CGRP. The image analysis was used for quantitative analysis and SAS software was used for statistics. 　Results　By aging, the bundle of CGRP-immunoreactive nerve became thin gradually, the numbers of CGRP-immunoreactive nerve branch decreased, the staining density of CGRP-immunoreactive nerve also descended and the distribution of CGRP-immunoreactive nerve fibers into predine was shorter. Image analysis showed that the intergral optical density, volume density, length density and line length of CGRP-immunoreactive nerve were all decreased by aging. The differences in volume density, length density and line length of CGRP-immunoreactive nerve were significant among different groups, but this significance was not found in parameter of integral optical density of CGRP-immunoreactive nerve.　Conclusions　The numbers, branches and extent of distribution of CGRP-immunoreactive nerve fibers decreased by age. The sensitivity of CGRP-immunoreactive nerve may also be lower by age.