摘要目的 探讨老年人外周血清可溶性凋亡相关因子(sFas)及其配体(FasL)水平的变化及其意义. 方法 采用双抗体夹心酶联免疫吸附法(ELISA),检测50例老年人血清sFas和FasL水平,与非老年人进行对照研究,并分析老年人血清sFas、FasL水平与心理应激以及应对方式之间的关系. 结果 老年组血清sFas水平为(10.6±2.8)μg/L,明显高于非老年组(7.5±2.2)μg/L,差异有统计学意义(P<0.01);FasL水平为(34.9±19.8)μg/L,明显低于非老年组(52.9±16.1)μg/L,差异有统计学意义(P<0.01).老年组sFas水平与心理应激成正相关(r=0.453,P<0.01);与积极应对方式成负相关(r=-0.535,P<0.01). 结论 老年人血清sFas、FasL水平异常,提示增龄增加了Fas介导的细胞凋亡,且与个体心理应激状况、应对方式有一定关系,这可能提示慢性应激是老年人免疫衰老的重要原因之一;同时老年人体内还可能存在着Fas/FasL的动态平衡机制.

abstractsObjective To investigate the changes and the clinical significance of serum concentration of soluble Fas (sFas) and Fas ligand (FasL) in the elderly. Methods Fifty elderly subjects and forty-seven adults were recruited. Serum concentration of sFas and EasL were detected by enzyme-linked immunosorbent assay (ELISA) . Psychosocial stress and coping styles were also evaluated. Results The sFas level of the elderly was significantly higher than that in control group,whereas the EasL level was decreased with ageing (P<0.01). A positive correlation between sFas level and psychosocial stress, and a negative correlation between sFas level and positive coping were also found (all P<0. 05). Conclusions Age-related changes occur in serum sEas and EasL levels.The interaction of sFas/FasL system with psychosocial stress and coping styles seems to play important roles in immunosenescence. Our results also suggest that during aging a subtle balance between sFas and FasL could exist.