摘要目的 观察小鼠肺脏基质细胞分泌的血管内皮生长因子(VEGF)对成熟树突状细胞(mDC)分化和功能及免疫耐受的影响.方法 建立肺脏基质细胞/树突状细胞共培养体系作为对照组,体系中加入VEGF抗体作为实验组(VEGF抗体组).反转录聚合酶链反应法检测肺脏基质细胞微环境中细胞因子的表达；透射电镜观察细胞形态.流式细胞术检测细胞表型；CCK-8法检测树突状细胞诱导T细胞增殖的能力.结果 VEGF抗体组树突状细胞表型CD86表达与对照组相比明显上调(P＜0.05),诱导T细胞增殖的能力差异无统计学意义(P＞0.05).结论 VEGF通过下调CD86的表达参与树突状细胞诱导免疫耐受的过程.

abstractsObjective To observe the effects of vascular endothelial growth factor(VEGF)from pulmonary stromal cells on differentiation and function of mature dendritic cells (mDC) and the immune tolerance. Methods Co-culture system of murine pulmonary stroma cells (MPSC)/mDC was established as control group,and VEGF-Ab add-on treatment to co-culture cells as experimental group.Cytokines secreted from pulmonary stroma cells were detected by RT-PCR.The expression of cellular phenotype and ability of the mDC-induced T cells reproductive activity were detected by flow cytometry and CCK-8,respectively.Results CD86 expression of VEGF-Ab group was higher than of control group (P ＜ 0.05).There was no difference in the ability of mDC-induced T cells reproductive activity between the two groups(P＞0.05).Conclusions VEGF might be involved in the regulation of immune tolerance by reducing expression of CD86 in dendritic cells.