摘要目的 探讨大鼠半胱氨酸蛋白酶9(Caspase-9)及热休克蛋白-90(HSP-90)的表达.方法 将200～250 g的SD雄性大鼠随机分为正常对照组、假手术组和局灶性脑缺血再灌注组,根据各组的再灌注时间,将每个组分为4个亚组,即6、24、48、和72 h四个亚组;采用线栓法制备大鼠局灶性脑缺血再灌注的损伤模型,行酶联免疫学检测(ELISA)法检测大鼠Caspase-9及HSP-90的表达的变化. 结果 ELISA法观察大鼠脑细胞Caspase-9、HSP-90含量的变化,Caspase-9及HSP-90表达在假手术组为弱表达,在局灶性脑缺血再灌注组的表达在24～48 h达高峰,72 h开始下降.假手术组与正常对照组相比结果差异无统计学意义.脑缺血再灌流后,随着时间的延长凋亡细胞数逐渐增加,至48 h达高峰;在缺血半暗带区,再灌流后神经细胞HSP-90和Caspase-9表达逐渐增强,到48h阳性细胞数目最多. 结论 脑缺血再灌流后神经细胞凋亡是一个动态的渐进过程.HSP-90和Caspase-9基因表达在介导细胞凋亡过程中起重要作用.

abstractsObjective To investigate the expression of Caspase-9 and heat-shock protein-90 (HSP 90) in rats after focal cerebral ischemia-reperfusion injury.Methods The male SD rats (200-250 g) were divided into three groups by the random number table: normal group, sham group and cerebral ischemia-reperfusion (CIR) group.Each group was sorted into four subgroups including group 6 h, group 24 h, group 48 h and group 72 h according to the reperfusion time.Suture-occluded method was adopted to prepare focal cerebral ischemia-reperfusion(CIR) injury in rat model.Enzymelinked immunosorbent assay (ELISA) method was used to detect the variations of Caspase-9 and HSP-90 expression in rats.Results The changes in Caspase-9 and HSP 90 expression in the brain cells were observed by ELISA method.The expression of Caspase-9 and HSP-90 was weakly expressed in sham group, and was at peak in CIR group within 24 h-48 h, then began to decline at 72 h after the reperfusion time.The differences in the expression of caspase-9 and HSP-70 between sham group and normal group were not statistically significant.Conclusions Apoptotic cells gradually increase along with reperfusion time and reach the peak at 48 h after cerebral ischemia-reperfusion.In ischemia half dark stripe, the expression of Caspase-9 and HSP 90 is increased in neuronal cells after cerebral ischemia-reperfusion, and the positive cells number is at peak at 48 h after cerebral ischemiareperfusion.Apoptosis of neuronal cells after cerebral ischemia and reperfusion is a dynamic evolutionary process.The expression of Caspase-9 and HSP 90 in nerve cells plays an important role in regulating cell apoptosis.