摘要目的 了解辽宁省乙型肝炎(乙肝)监测病例HBV基因型分布及其主蛋白抗原主要亲水区(MHR)氨基酸(aa)位点变异情况.方法 采集辽宁省监测试点县区乙肝病例血清,提取HBV DNA并进行序列扩增,测序得到S基因序列,利用生物学软件比较分析.结果 共得到HBVS基因序列81条,基因型分布B型为8.64％(7/81)、C型为86.42％(70/81)、D型为4.94％(4/81).S基因MHR aa位点变异率为4.87％,T126I变异率最高,为8.64％.HBV MHR变异株总流行率为49.38％(40/81),其中B型为42.86％(3/7),C型为47.14％(33/70),D型为100％(4/4).在不同年龄、性别、基因型和抗-HBc IgM(+/-)分类比较中,差异无统计学意义,但ALT正常组(＜43 IU/L)该流行率显著高于ALT异常组(≥43 IU/L).结论 辽宁省乙肝监测病例中,基因型分布以C型为主,B型次之,偶见D型.HBV MHR aa位点变异与既往研究结果一致,仍为今后乙肝监测重点.

abstractsObjective To understand the genotype and the mutation of amino acid (aa) in the major hydrophilic region (MHR) of hepatitis B virus (HBV)among the hepatitis B cases under surveillance in Liaoning province.Methods The serum samples were collected from hepatitis B cases under surveillance in Liaoning.The complete S gene of HBV were amplified and sequenced.The aa sequences were analyzed with bioinformatics software.Results A total of 81 sequences of S gene of HBV were ontained,including 7 sequences of genotype B (8.64％),70 sequences of genotype C (86.42％) and 4 sequences of genotype D (4.94％).The mutation rate of aa of MHR of S gene was 4.87％.The mutation rate of T126I was highest (8.64％).The overall prevalence of mutant strain of MHR was 49.38％(40/81),and it was 42.86％ for genotype B(3/7),47.14％ for genotype C (33/70) and 100％ for genotype D (4/4).Statistical analysis revealed that there was no age,sex,genotypes and anti-HBc IgM (+/-) specific significant differences in aa mutant strains prevalence,while the difference between ALT abnormal group (ALT ≥ 43 IU/L) and ALT normal group (ALT ＜ 43 IU/L) was significant (P＜0.05).Conclusion Among the hepatitis B cases under surveillance in Liaoning,HBV genotype C predominant,followed by genotype B and genotype D.The mutation of aa in MHR of HBV detected in this study was consistent with previous research results.It is necessary to strengthen the surveillance for HBV mutation to provide accurate information for the development of hepatitis B prevention and control measures.