摘要目的 了解云南省2个边境地区新报告HIV感染者HIV-1基因亚型分布特征.方法 2015年11月至2016年10月在云南省德宏傣族景颇族自治州(德宏州)和红河哈尼族彝族自治州(红河州)边境地区,连续纳入年龄≥18岁新报告HIV感染者共233例,提取病毒RNA,扩增pol和env基因区,进行基因分型检测和序列分析.结果 233份标本共有146份获得HIV-1基因亚型.德宏州有8种HIV-1基因亚型,以独特重组型(URFs)为主(52.8％,57/108),其中中国籍的URFs占56.8％ (21/37),缅甸籍的占50.7％ (36/71);红河州有4种HIV-1基因亚型,以CRF01_AE为主(71.1％,27/38),其中越南籍的占81.0％(17/21),中国籍的占58.8％(10/17).2个州HIV-1基因亚型总体分布的差异有统计学意义(x2=61.072,P＜0.001).结论 云南省2个边境地区新报告HIV感染者HIV-1基因亚型分布差异较大,应对中国籍和外国籍HIV感染者采取有针对性的防治措施.

abstractsObjective To explore the features of distribution on HIV-1 gene subtypes among newly reported HIV/AIDS cases in the border areas of Yunnan province.Methods A total of 233 newly reported HIV/AIDS cases aged 18 or more were consecutively included in the border counties of Dehong Dai and Jingpo autonomous prefecture (Dehong prefecture),Honghe Hani and Yi autonomous prefecture (Honghe prefecture) of Yunnan province from November 2015 to October 2016.HIV-1 RNA was extracted with pol and env genes amplified.HIV-1 gene subtypes were determined through phylogenetic analysis.Results A total of 146 out of 233 specimens were genotyped successfully.HIV-1 was found to have had 8 gene subtypes in Dehong prefecture,with the unique recombinant forms (URFs) as the predominant (52.8％,57/108) type,including 56.8％ (21/37) of the cases with Chinese ethnicity and another 50.7％ (36/71) were Myanmar citizens.Four HIV-1 gene subtypes were detected in Honghe prefecture,with CRF01_AE as predominant (71.1％,27/38),including 81.0％ (17/21) Vietnamese and 58.8％ (10/17) Chinese.Differences on the distribution of HIV-1 gene subtypes were seen statistically significant between Dehong prefecture and Honghe prefecture (x2=61.072,P＜0.001).Conclusions The distribution of HIV-1 gene subtypes showed big difference in the two border areas of Yunnan province,suggesting that both Chinese or non-Chinese citizens living in the area should be taken good care of,in terms of HIV/AIDS prevention and control.