摘要目的 构建一种能够建立免疫嵌合现象的带血管髂骨移植动物模型.方法 2015年12月至2016年3月采用SD大鼠进行实验.实验分为3组:实验组、阳性对照组和阴性对照组.实验组受者为10只雌性SD大鼠,对其实施改良的带血管髂骨移植手术,供者为10只雄性SD大鼠;阳性对照组为4只无处理雄性SD大鼠,阴性对照组为4只无处理雌性SD大鼠.术后第28天PCR检测Y染色体性别决定区(sex determining region of the Y,SRY)基因,流式细胞术动态监测CD4+/CD8+T细胞比值,酶联免疫吸咐试验动态监测IL-2水平,影像学和病理学检测移植髂骨.结果 实验组10只大鼠行移植手术,其中7只皮瓣成活,皮瓣成活率70％.实验组10只受者大鼠中4只术后检测出SRY基因,微计算机断层扫描技术(micro computed tomography,Micro-CT)扫描显示移植髂骨骨密度降低,CD4+/CD8+T细胞比值和IL-2水平与正常大鼠相比差异无统计学意义(P＞0.05);病理结果显示移植髂骨生长正常.结论 通过改良的的带血管髂骨移植手术,成功在雌性大鼠体内检测出SRY基因,证明了其具备建立嵌合现象的能力,且在免疫抑制剂的干预下,大鼠免疫状态良好,为进一步探索嵌合现象与免疫耐受提供了基础.

abstractsObjective To construct an animal model of vascularized iliac bone transplantation which could establish the immune chimerism.Methods The experiment was divided into three groups.In experiment group we slected the male SD rats as donors and the female SD rats as recipients.Then the experiment group was performed the improved vascularized ilium transplantation operation.The positive group was male SD rats without handling.The negative group was female SD rats without handling.On the twenty-eighth day after surgery, the rat SRY gene was detected by PCR.Dynamically monitoring the changes of the ratio of CD4+/CD8+ T cells by flow cytometry after operation.Dynamically monitoring the changes of the levels of serum IL-2 by enzyme linked immunosorbent assay after operation.Imaging and pathology were used to detect the transplanted iliac bone.Results Ten transplantations were performed in the experiment group and 7 flaps survived with a successful rate of 73 ％.SRY gene was detected in female rats that receive the male rats'iliac bone transplantation by PCR in the experiment group.After Micro-CT scanning and three-dimensional reconstruction, it showed that the transplanted iliac bone mineral density was decreased.Comparing rats that establish the chimerism with normal rats, the ratio of CD4 +/CD8 + T cells and the levels of serum IL-2 had no difference.Pathological results showed that the transplanted iliac bone of rats with chimerism were normal and the rats without chimerism were necrotic.Conclusions From the improved vascularized ilium transplantation operation, we successfully detected the SRY gene in female rats which received the male rats' iliac bone transplantation.It proved that the improved vascularized ilium transplantation operation could establish the chimerism.Through the immunosuppressant, the immune status of the rat after operation was well.