摘要目的 评价异丙酚对LPS诱导BV-2小胶质细胞IL-1β和TNF-α释放的影响及Toll样受体4(TLR4)在其中的作用.方法 将体外培养的BV-2小胶质细胞接种于96孔培养板中,采用随机数字表法,将其随机分为4(n=12):对照组、LPS组、异丙酚组和LPS+异丙酚组.LPS组加入LPS1μg/ml孵育24h;异丙酚组加入异丙酚30 μmol/L孵育24 h;LPS+异丙酚组同时加入LPS 1 μg/ml和异丙酚30 μmol/L孵育24h.于孵育6h时,采用ELISA法检测细胞上清液TNF-α浓度,以此反映TNF-α的释放量,采用RT-PCR法测定TLR4 mRNA表达；于孵育24h时,采用ELISA法检测细胞上清液IL-1β浓度,以此反映IL-1β的释放量,采用Western Blot法检测TLR4蛋白表达.结果 与C组比较,LPS组和LPS+异丙酚组IL-1β和TNF-α的释放量升高,TLR4 mRNA及其蛋白表达上调(P＜0.05)；与LPS组比较,LPS+异丙酚组IL-1β和TNF-α的释放量降低,TLR4 mRNA及其蛋白表达下调(P＜0.05).结论 异丙酚可抑制LPS诱导BV-2小胶质细胞IL-1β和TNF-α的释放,其机制与抑制TLR4的表达有关.

abstractsObjective To investigate the effect of propofol on IL-1β and TNF-α release from BV-2 microglia cells induced by lipopolysaccharide (LPS) and the role of Toll-like receptor 4 (TLR4).Methods BV-2 microglia cells were seeded in 96-well plates and randomly divided into 4 groups ( n =12 each):control group,LPS group,propofol group (group P) and LPS + propofol group.In group LPS,the cells were incubated with LPS 1 μg/ml for 24 h.In group P,the cells were incubated with propofol 30 μmol/L for 24 h.In group LPS + propofol,the cells were incubated with LPS 1 μg/ml and propofol 30 μmol/L for 24 h.The concentrations of TNF-α ( at 6 h of incubation) and IL-1β (at 24 h of incubation) in the supernatant were detected by ELISA.TLR4 mRNA expression was detectedat at 6 h of incubation by RT-PCR.TLR4 protein expression was detected at 24 h of incubation by Western blot.Results Compared with control group,IL-1β and TNF-α release was significantly increased,and the expression of TLR4 mRNA and protein up-regulated in groups LPS and LPS + propofol ( P ＜ 0.05).Compared with group LPS,IL-1β and TNF-α release was significantly decreased,and the expression of TLR4 mRNA and protein down-regulated in group LPS + propofol (P ＜ 0.05 ).Conclusion Propofol can inhibit IL-1β and TNF-α release from BV-2 microglia cells induced by LPS and inhibition of TLR4 expression may be involved in the mechanism.