摘要目的 评价腹腔注射不同剂量异丙酚对哮喘小鼠气道高反应性、肺部炎症以及Th1/Th2平衡的调节作用.方法 健康雌性BALB/c小鼠100只,6～8周龄,体重18 ～ 20 g,采用随机数字表法,将小鼠分为5组(n=20)∶对照组(C组)、哮喘组(A组)、低剂量异丙酚组(50 mg/kg i.p.,LP组)、中等剂量异丙酚组(100 mg/kg i.p.,MP组)和高剂量异丙酚组(150 mg/kg i.p.,HP组).使用卵清蛋白(OVA)构建过敏性哮喘模型小鼠,腹腔注射-诱导过敏性哮喘模型.各组随机取10只于模型构建成功后24 h即高剂量麻醉处死,行心脏取血及支气管肺泡灌洗,以ELISA法检测血清OVA特异性IgE和支气管肺泡灌洗液中特征性细胞因子IL-4、IL-5、IFN-γ浓度,并行细胞计数/分类计数.各组剩余的10只于模型构建成功后24h行肺功能检测和取肺进行HE染色,比较各组小鼠气道反应性和肺组织的病理组织学评分.结果 腹腔注射异丙酚(LP组和MP组)可明显地抑制哮喘小鼠的气道高反应性.不同剂量异丙酚干预组(LP组、MP组和HP组)均可明显抑制哮喘小鼠肺组织中嗜酸性粒细胞浸润,降低IL-4、IL-5和血清OVA特异性IgE水平,上调IFN-γ/IL-4比值.结论 异丙酚能够有效抑制哮喘小鼠肺部炎症,上调Th1/Th2比值,改善肺功能,发挥气道保护作用.

abstractsObjective To investigate the immunoregulatory effects of propofol on airway hyperresponsiveness,airway inflammation and Thl/Th2 ratio in the asthmatic mice.Methods One hundred female BALB/c mice,aged 6-8 weeks,weighing 18-20 g,were randomly divided into 5 groups (n =20,each):control group (normal saline i.p.,group C),asthma group (group A),low-dose propofol (50 mg/kg i.p.,group LP),medium-dose propofol (100 mg/kg i.p.,group MP) and high-dose propofol (150 mg/kg i.p.,group HP).Mice of groups A,LP,MP and HP were sensitized with ovalbumin (OVA),mice of group C were sensitized with normal saline.24 h after the last challenge,animals were sacrificed by lethal dose of pentobarbital sodium.Blood and bronchoalveolar lavage fluid (BALF) were collected for determination of serum OVA-specific IgE and the levels of cytokines (IL-4,IL-5 and IFN-γ) in the BALF.Airway responsiveness was measured by the forced-oscillation technique and histological inflammation scores were measured by staining with hematoxylin and eosin.Results Propofol (group LP and group MP) attenuated airway hyperresposiveness to the muscarinic agonist methacholine in OVA-induced asthma.Different doses of propofol (group LP,group MP and group HP) decreased eosinoplils influx in lungs.In addition,propofol treatment reduced expression of IL-4,IL-5 and serum OVA-specific IgE and increased the ratio of IFN-γ/IL-4.Conclusion The study demonstrates a potential protective value of propofol in alleviating airway inflammation,up-regulating Th1/Th2 ratio and attenuating airway hyperresposiveness in the asthmatic mice.