摘要目的 评价七氟醚对阿尔茨海默病小鼠认知功能的影响.方法 雄性淀粉样前体蛋白(APP)/早老素1双基因小鼠20只,体重30～ 40 g,7月龄,采用随机数字表法分为2组(n=10):七氟醚组(Sev组)和对照组(C组).Sev组吸入3％七氟醚4h,C组吸入30％氧气4h.吸入七氟醚或30％氧气后1个月,进行连续多次被动逃避实验.随后处死小鼠取海马,采用免疫组化法和Western blot法分别检测小鼠海马Aβ斑块数、APP表达和Tau(S396)的磷酸化水平.结果 与C组比较,Sev 组记忆潜伏期缩短,海马Aβ斑块数增加,Tau(S396)磷酸化水平升高,APP表达上调(P＜0.05).结论 七氟醚可降低阿尔茨海默病小鼠认知功能.

abstractsObjective To evaluate the effect of sevoflurane on cognitive function of mice with Alzheimer's disease.Methods Twenty male mice carrying mnutations in amyloid precusor protein (APP) and presenilin 1 genes,weighing 30-40 g,aged 7 months,were divided into either sevoflurane group (group Sev) or control group (group C),with 20 mice in each group.Mice inhaled 3％ sevoflurane for 4 h in group Sev,and mice inhaled 30％ oxygen for 4 h in group C.At 1 month after inhaling sevoflurane or oxygen,the mice underwent continuous multiple-trail inhibitory avoidance training.The mice were then sacrificed and hippocampi were isolated for determination of the number of Aβ plaques (by immunohistochemistry) and expression of APP and Tau (S396) phosphorylation (by Western blot).Results Compared with group C,the memory lateucy was significantly shortened,the number of Aβ plaques was increased,the phosphorylation of Tau (S396) was increased,and the expression of APP was up-regulated in group Sev (P＜0.05).Conclusion Sevoflurane can decrease the cognitive function of mice with Alzheimer's disease.