摘要目的:评价人类肠道 Dorea菌在属、种水平对肥胖患者糖代谢异常的作用及预测价值。 方法:本研究纳入体重指数(BMI)≥30 kg/m 2的肥胖患者113例，招募正常对照人群105例，采用横断面研究方法比较肥胖合并不同程度糖代谢异常患者以及正常对照人群的临床指标，肠道 Dorea菌属、种水平差异。相关性分析探索肠道 Dorea菌属与临床代谢指标的相关性，使用受试者工作(ROC)曲线分析评估人体肠道 Dorea菌属在属、种水平的丰度对肥胖患者发生糖代谢异常的早期筛查及预测能力。 结果:(1) ANOVA分析显示肥胖组代谢相关临床指标明显高于体重正常的对照组。按糖耐量分层分析，糖尿病患者与糖耐量受损及糖耐量正常人群的体重、BMI、腰臀比均无显著性差异。(2)肥胖人群肠道 Dorea菌属、 Dorea formicigenerans丰度显著高于正常对照人群，但在肥胖合并糖耐量受损及糖尿病患者中 Dorea formicigenerans丰度低于肥胖糖耐量正常人群。肥胖人群中进行相关性分析显示，人类肠道 Dorea formicigenerans与口服葡萄糖耐量试验(OGTT)2 h血糖、HbA 1C呈负相关，与HOMA-β呈正相关。 Logistic回归分析显示，校正年龄、性别等混杂因素后， Dorea formicigenerans菌种是肥胖患者糖代谢异常的独立保护因素。(3) ROC曲线分析显示，对总体肥胖人群而言， Dorea formicigenerans对糖代谢异常发生的筛选能力AUC值为0.73。 结论:Dorea菌属、 Dorea formicigenerans菌种是肥胖患者糖代谢的保护因素，对肥胖患者合并糖代谢异常具有良好预测能力。可用于早期筛查和监测．

abstractsObjective:To evaluate the role of Dorea in glucose intolerance of people with obesity. Methods:This study recruited 113 young individuals with obesity and varying degree of glucose tolerance [body mass index(BMI)≥30 kg/m 2] and 105 controls, comparing the metabolic phenotypes and Dorea abundance. Correlation analysis and ROC analysis were performed to assess the association between Dorea and clinical parameters and its predictive role in predicting glucose intolerance. Results:(1) Metabolic parameters were higher in obesity group than the control group. There was no difference in body weight, BMI and WHR among subgroups classified by glucose tolerance in people with obesity. (2) The abundance of Dorea, Dorea formicigenerans were higher in obese individuals, however showing a downward trend in accordance with glucose intolerance. The abundance was inversely associated with OGTT-2 h plasma glucose and HbA 1C, while positively associated with HOMA-β. Logistic regression demonstrated that Dorea formicigenerans was an independent protective factor after adjusting confounders such as age and gender in the prevention of glucose intolerance. (3) ROC analysis exhibited that the AUC values of Dorea formicigenerans was 0.73 in the total population. Conclusion:Dorea and Dorea formicigenerans exert protective effect on glucose metabolism in obese subjects. The abundance of Dorea and Dorea formicigenerans can be used as predictors of glucose intolerance risk in obese subjects, which facilitate the early screening and monitoring.