摘要目的 研究慢性淋巴细胞白血病(CLL)正调节基因1(CLLU1)在CLL患者的表达特征,探讨CLLU1在CLL预后中的意义.方法 采用实时定量RT-PCR(qRT-PCR)检测41例CLL患者外周血标本CLLU1的表达.以3-磷酸甘油醛脱氰酶(GAPDH)为内参、2~(-△Ct)方法计算CLLU1的相对定量值.组间对比采用秩和检验,Spearman相关分析CLLU1表达水平与性别、年龄、临床分期、IgVH基因突变、CD_(38)和ZAP-70蛋白的关系.结果 qRT-PCR标准曲线相关系数R值均>0.99,敏感度可以检测到102个拷贝/μgRNA,批内差、批间差分析结果其变异系数(CV)均<5%.41例患者CLLU1表达水平中位数为0.139(0～5256.912).CLLU1表达水平与临床分期、IgVH基因突变、CD_(38)密切相关,Binet B+C期患者CLLU1表达水平明显高于Binet A期患者(P=0.040),无IgVH突变患者的CLLU1表达水平高于IgVH突变患者(P=0.021),CD_(38)阳性患者中CLLU1表达水平较阴性者高(P=0.045).结论 采用qRT-PCR方法检测CLLU1表达敏感可靠.CLLU1表达水平与CLL临床分期和重要预后因素IgVH基因突变及CD_(38)密切相关,并可较准确地预测IgVH突变情况,在CLL的预后中具有重要价值.

abstractsObjectives To investigate the expression level of chronic lymphocytic leukemia(CLL) up-regulated gene 1(CLLU1)mRNA in CLL and its prognostic value in CLL. nethods Real-time quantitative RT-PCR(qRT-PCR)was performed on 41 CLL patients using Taqman probe system.Correlation of CLLU1 expression ratio with other prognostic factors was carried out using Spearman correlation coefficient.Results The correlation coefficients of the standard curves in qRT-PCR were above 0.99.The coefficients of variation(CV)of interrun assay and intrarun assay were<5%and the sensitivity reached 12.copies/μg RNA.The median CLLU1 mRNA expression level was 0.139(0-5256.912)in 41 CLL patients.CLLU1 expression was significantly associated with Binet stages(P=0.040)and IgVH mutation status(P=0.021).CLLU1 expression was also associated with CD_(38) expression(P=0.045).Conclusion qRT-PCR assay is reliable and sensitive.CLLU1 mRNA expression significantly correlates with clinical stages,IgVH mutation status and CD_(38) expression and might be a prognostic maker of CLL.