摘要为了研究芳香维甲酸乙酯(Ro13-6298)对黑素细胞黑素合成的影响,为应用维甲酸治疗色素沉着性皮肤病提供实验依据.体外培养6例人正常黑素细胞,每日分别经100mJ/cm 2的中波紫外线(UVB)照射,每次照射后立即加入不同浓度(10 -9～10 -5mol/L)的Ro13-6298,于第2、4、6天分别用多巴氧化速率法和NaOH溶解法测定细胞酪氨酸酶活性和黑素含量.结果:每日用100mJ/cm 2的UVB连续照射培养的黑素细胞,照射4天后细胞酪氨酸酶活性和黑素含量显着高于非照射组(<i>P</i><0.05～0.01);在每次UVB照射后加入Ro13-6298,细胞酪氨酸酶活性和黑素含量则与非照射组无显着性差异;在非照射组未发现Ro13-6298对细胞黑素合成有明显影响作用.结论:反复小剂量UVB照射能通过提高酪氨酸酶活性来实现对培养黑素细胞黑素合成的诱导作用;Ro13-6298在较低浓度能抑制UVB对黑素细胞黑素合成的诱导作用.

abstractsThe purpose of this study is to invest the effect of arotinoid ethylester (Ro13-6298) on ultraviolet B(UVB)-induced melanogenesis of human normal melanocytes (HNM) and provide experimental evidence for treating pigmented diseases of skin. HNM taken from 6 normal persons cultured in vitro were treated with 100mJ/cm 2/day and/or different concentrations (10 -9～10 -5mol/L) of Ro13-6298 respectively, Ro13-6298 were added immediately after each irradiation. 2, 4 and 6 days after the irradiation, tyrosinase activity was estimated by measuring the rate of oxidation of L-DOPA, and melanin content by the value of optical density absorbance of NaOH treated HNM. Results showed that tyrosinase activity and melanin content of treated HNM after 4-day-UVB irradiation increased significantly (<i>P</i>&lt;0.05～0.01) than those of non-irradiated cells, but there was no statistical difference between the non irradiated cell group and Ro13-6298 treatment group. Little effect was found on the melanin synthesis in the non-irradiated HNM treated with Ro13-6298 (10 -9～10 -5). Conclusion: the melanogenesis of cultured HNM can be induced by repeated small dose of UVB irradiations through increasing tyrosinase activity, Ro13-6298 can inhibit melanogenesis induced by UVB irradiation on HNM at lower concentration in vitro.