摘要目的方法结果结论> 研究蕈样肉芽肿 (MF)和S??ary综合征 (SS)患者淋巴细胞DNA的不稳定性.方法采用彗星测定 (Comet Assay)或称为单一细胞凝胶电泳 (The single cell gel electrophoresis SCGE)研究受X线引起MF和SS患者外周血淋巴细胞、皮肤损害的淋巴细胞培养建立的T细胞系的DNA损伤和修复.以正常人淋巴细胞为对照组.<b>结果</b> MF和SS患者淋巴细胞DNA有明显的不稳定性,且很难修复,与对照组差异有显著性.结论MF和SS患者淋巴细胞的DNA表现明显的不稳定性和修复障碍,提示MF和SS是从一种良性皮肤病发展成恶性肿瘤.

abstractsObjectiveTo study the DNA instability of the peripheral blood lymphocytes and T-cell line from skin lesions of mycosis fungoides(MF) and S?ary's syndrome (SS).MethodsThe Comet assay,which was also called as SCGE (the single cell gel electrophoresis),was used to detecting the primary DNA damage at the level of single cell in specimens.The DNA damage of X-ray-irradiated peripheral blood lymphocytes and T lymphocytes from skin lesions,and its repair in lymphoma patients were studied,and compared with those in control persons.ResultsA significantly increased DNA instability in the majority of lymphocytes from the blood and skin lesions of MF and SS patients was observed,and reduced DNA repair of X-ray irradiated lymphocytes was found,when compared with the lymphocytes from healthy individuals.ConclusionThe DNA stability and repairment of both blood and skin-homing lymphocytes from patients with MF and SS are significantly impaired,which suggests that MF and SS could develop from a clinically benign skin disease into an overt lymphoma.