摘要目的 了解广西性病门诊泌尿生殖道沙眼衣原体感染率和基因分型状况及相关因素.方法 对598例性病门诊患者的泌尿生殖道标本经质粒PCR筛选后, 阳性者用巢式PCR扩增沙眼衣原体ompl基因片段,酶切ompl扩增产物,对沙眼衣原体各临床株ompl基因进行限制性片段长度多态性(RFIJP)分析,将临床株进行基因分型.结果 在598例性病门诊患者中,经PCR检测,83例沙眼衣原体阳性,感染率为13.9%,感染率在性别之间差异无统计学意义.阳性者的DNA进行RFLP分析,基因分型发现,E型占27.7%,F型占20.5%,D型占13.2%和G型占12.0%.基因型在不同性别、年龄和婚姻组之间的分布差异无统计学意义.E型和F型比其他型别更不易出现临床症状,而G型80%的病例出现下腹痛的表现.结论 广西性病门诊患者中存在一定比例的沙眼衣原体感染和多种基因型分布,ompl基因的多态性分析可以作为沙眼衣原体分子流行病学研究的工具,同时应加强对泌尿生殖道沙眼衣原体感染的筛查、控制及感染者的随访.

abstractsObjective To determine the prevalence and genotype profiles of urogenital Chlamydia trachomatis and their related factors in patients attending sexually transmitted diseases (STD) clinics in Guangxi Zhuang Autonomous Region. Methods C. trachomatis was screened by a plasmid PCR in 598 patients attending STD clinics. Then, positive specimens underwent nested-PCR to amplify the major outer membrane protein 1 (ompl) gene. The amplicons of ompl gene were digested by restriction endonucleases Alu I plus Hinf I and Cfol . C. trachomatis was differentiated according to the restriction fragment length polymorphism (RFLP) patterns. Results Out of the 598 samples, 83 were positive for plasmid-PCR. The prevalence of chlamydial infection was 13.9% with no significant difference between male and female patients. Nested-PCR based RFLP analysis showed that genotype E amounted to 27.7% (23/83), F 20.5% (17/83), D 13.2%(11/83), G 12.0%(10/83), K 7.2%(6/83), H 4.8%(4/83), I 3.6% (3/83), J 3.6%(3/83)and uncertain types 7.2% (6/83). Visible symptoms were observed less frequently in infections with C. trachomatis genotypes E and F compared with the other genotypes, while low abdominal pain occurred in 80% of infections with type G. Conclusions A certain proportion of out-patients attending STD clinic are infected with various types of C. trachomatis in Guangxi Zhuang Autonomous Region. The polymorphism of ompl gene may serve as a useful tool in molecular epidemiological studies of C. trachomatis.