摘要目的 探讨T细胞受体γ(TCR-γ)基因重排在蕈样肉芽肿(MF)诊断中的意义,寻找一种较为敏感的基因诊断方法.方法 以TCR-γ基因的Vγ8、Vγ9、Vγ10、Vγ11、Jγ1-2为引物,利用PCR-琼脂糖凝胶电泳方法检测共50份标本的基因重排情况,其中30例MF患者的皮损标本33份,淋巴结标本2份;15例炎症性皮肤病患者的15份皮损标本.结果 30例MF患者的33份皮肤标本,共有29份检测到TCR-γ基因单克隆性重排的条带,总阳性率为88%.15份炎症性疾病皮肤标本中,共有5份检测到TCR-γ基因单克隆性重排的条带,阳性率为33%.结论 TCR-γ基因重排检测可以应用于MF的诊断与鉴别诊断,但只能作为一种辅助诊断方法.

abstractsObjective To assess the diagnostic significance of T cell receptor gamma gene rearrangemerits in mycosis fungoides (MF), so as to develop a sensitive diagnosis tool. Methods A total of 50 specimens were collected, including 33 skin lesion specimens and 2 lymph specimens from 30 patients with MF,15 skin lesion specimens from 15 patients with inflammatory dermatoses. PCR was performed with specific primers targeting TCR V gamma 8, 9, 10, 11 to detect T cell receptor gamma gene rearrangement. Results Monoclonai rearrangements of TCR gene was observed in 88% (29/33) of specimens from patients with MF and 33% (5/15) of samples from patients with inflammatory dermatoses. Conclusions The detection of TCR gene rearrangements, as an ancillary test, is useful in the diagnosis and differential diagnosis of MF.