摘要目的 探讨丹参酮ⅡA磺酸钠(STS)外用对动物模型皮脂腺增生的影响。方法 选用成年雄性金黄地鼠侧腹皮脂腺斑作为动物模型，采用自身对照法，分别予丹参酮ⅡA磺酸钠、生理氯化钠溶液外涂一侧皮脂腺斑，一天3次，随机分用药0、10、20、30d4个时间组。在各时间点用游标卡尺测定两侧皮脂腺斑的面积。HE染色法观察皮脂腺斑组织结构的变化，取组织切片用免疫组化方法检测皮脂腺细胞增殖细胞核抗原(PCNA)的表达，TUNEL法检测皮脂腺细胞的凋亡情况。结果 用药前，两侧皮脂腺斑大小比较差异无统计学意义(P＞0.05)，皮脂腺结构完整，排列紧密，皮脂腺细胞的增殖与凋亡比较差异均无统计学意义(P＞0.05)。随着用药时间的延长，与生理氯化钠溶液对照侧比较，STS侧金黄地鼠皮脂腺斑的面积缩小(P＜0.05)；皮脂腺数目减少，体积变小，排列疏松，用药至30 d时，皮脂腺呈明显萎缩状态；皮脂腺细胞PCNA表达明显下调(P＜0.01)，用药至10 d、20 d时更为明显(P＜0.01)；皮脂腺细胞凋亡亦增加(P＜0.01)，用药至20 d时凋亡显著(P＜0.01)，且皮脂腺中央部细胞的凋亡较周围更为明显。结论 丹参酮ⅡA磺酸钠能缩小金地鼠皮脂腺斑的面积，改变皮脂腺斑的显微结构，可抑制皮脂腺增生。

abstractsObjective To observe the effect of topical sulfotanshinone sodium (STS) on sebaceous hyperplasia in animal models. Methods The sebaceous gland spots of adult male Syrian hamster flank organ served as the animal model. Sulfotanshinone sodium (0.5％) was applied to sebaceous gland spots in the right flank organ thrice daily, while those in the left were treated with normal saline as control. Parameters were examined before, 10 days, 20 days and 30 days after the beginning of the topical treatment. A vernier caliper was utilized to measure the size of sebaceous gland spots, hematoxylin and eosin (HE) staining to observe the structure of sebaceous glands, immunohistochemistry to determine the expression of proliferating cell nuclear antigen (PCNA) in sebaceous gland cells, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to assess the apoptosis of sebaceous gland cells. Results At the baseline, no significant difference was observed in the size of sebaceous gland spots or in the proliferation and apoptosis of sebaceous gland cells between the two sides of flank organ (all P ＞ 0.05), with tightly arranged intact sebaceous glands. Compared with normal saline, sulfotanshinone sodium significantly reduced the size of sebaceous gland spots (P ＜ 0.05). Sebaceous glands were loosely arranged with decreased quantity and volume and obviously atrophic on day 30 in the right flank organ of hamsters. A decrease was observed in the expression of PCNA in sulfotanshinone sodium treated sebaceous gland cells compared with those treated with normal saline (P ＜ 0.01 ), which was more striking on day 10 and 20 (both P ＜ 0.005). Sulfotanshinone sodium also induced an enhancement of apoptosis in sebaceous gland cells (P ＜ 0.01 ), which was more apparent on day 20 (P ＜ 0.005 ), and the degree of apoptosis was higher in the central area than in the peripheral area of sebaceous glands. Conclusion Sulfotanshinone sodium can reduce the size and alter the microstructure of sebaceous gland spots, and inhibit the hyperplasia of sebaceous glands.