摘要目的 鉴定并分析白念珠菌氟康唑敏感株与耐药株的差异表达膜蛋白质.方法 以同一亲本来源的白念珠菌氟康唑敏感株CA-3和耐药株CA-16为研究对象,以双向聚丙烯酰胺凝胶电泳(2D-PAGE)技术分析菌株之间膜蛋白图谱的差异,以基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF/TOF-MS)鉴定表达差异的蛋白质,通过白念珠菌蛋白质数据库进行检索分析.结果 白念珠菌氟康唑耐药株较敏感株有22种差异表达蛋白质点,其中有6种蛋白质上调,为Adh1p、Csp37p、Pgk1p、Pgk1p和2个未命名蛋白质(gi227305312、gi53954641);16种蛋白质下调,分别为Aco1p、Aco1p、Hsp78p、Gut2p、Sdh12p、Ilv2p、Ndh51p、Ndh51p、Atp1p、Pda1p、Srb1p、Idh1p、Tdh1p、Cyt1p、Cox4p、Cox13p.结论 白念珠菌氟康唑耐药株较敏感株差异膜蛋白主要涉及能量代谢、线粒体功能等.

abstractsObjective To identify and analyze plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strains.Methods Two C.albicans strains from a same parent,including the fluconazole-sensitive C.albicans strain CA-3 and fluconazole-resistant C.albicans strain CA-16,served as the subject of this study.Plasma membrane proteins were isolated from both of the C.albicans strains,and subjected to two-dimensional polyacrylamide gel electrophoresis analysis for the screening of differentially expressed proteins,which were then identified by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry.The resultant data were searched against a protein database for C.albicans.Results Twentytwo proteins were identified to be differentially expressed between the fiuconazole-resistant and-sensitive C.albicans strain.Of them,6 proteins (Adh1p,Csp37p,Pgk1p,Pgk1p and 2 unnamed proteins,i.e.,gi227305312and gi53954641) were highly expressed,while 16 proteins (Aco1p,Aco1p,Hsp78p,Gut2p,Sdh12p,Ilv2p,Ndh51p,Ndh51p,Atp1p,Pda1p,Srb1p,Idh1p,Tdh1p,Cyt1p,Cox4p,Cox13p) were lowly expressed in the fluconazole-resistant C.albicans strain compared with the fluconazole-sensitive strain.Conclusion The plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strain are mainly implicated in energy metabolism and mitochondrial function.